Immunomagnetic assay combined with CdSe/ZnS amplification of chemiluminescence for the detection of abscisic acid  

作　　者：ZHOU GuoHua, WANG Ping, YUAN Ju, QIU Ting & HE ZhiKe Key Laboratory of Analytical Chemistry for Biology and Medicine, Ministry of Education College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China 

出　　处：《Science China Chemistry》2011年第8期1298-1303,共6页中国科学（化学英文版）

基　　金：supported by the National Natural Science Foundation of China (21075093 & 90717111);the Science Fund for Creative Research Groups of the National Natural Science Foundation of China (20921062);the National Key Scientific Program-Nanoscience and Nanotechnology (2011CB933600);the National Basic Research Program of China (2007CB714507)

摘　　要：Abscisic acid (ABA) is an important plant hormone. It plays a key role in regulating plant responses to abiotic stress and in controlling seed germination, growth, and stomatal aperture. A rapid, sensitive analytical method for the ABA detection is urgently required for further investigation of ABA signaling. In this work, an immunomagnetic assay combined with CdSe/ZnS amplification of chemiluminescence has been developed for the detection of ABA. The result could be read out in 30 min at least, with the simplified procedure of immunomagnetic assay. Under the optimized condition, a linear range from 1 pM to 10 nM was obtained. An unexpected result induced by the dose hook effect was discussed. This method provided the high selectivity for ABA over other components that might be contained in real samples.Abscisic acid （ABA） is an important plant hormone. It plays a key role in regulating plant responses to abiotic stress and in controlling seed germination, growth, and stomatal aperture. A rapid, sensitive analytical method for the ABA detection is urgently required for further investigation of ABA signaling. In this work, an immunomagnetic assay combined with CdSe/ZnS amplification of chemiluminescence has been developed for the detection of ABA. The result could be read out in 30 min at least, with the simplified procedure of immunomagnetic assay. Under the optimized condition, a linear range from 1 pM to 10 nM was obtained. An unexpected result induced by the dose hook effect was discussed. This method provided the high selectivity for ABA over other components that might be contained in real samples.

关 键 词：abscisic acid immunomagnetic assay CHEMILUMINESCENCE CDSE/ZNS 

分 类 号：Q503[生物学—生物化学]