TiO_2-functionalized mesoporous materials for sensitive analysis of multi-phosphopeptides  

作　　者：GUO WeiChao WAN JingJing QIAN Kun YU ChengZhong KONGJiLie YANG PengYuan LIU BaoHong 

机构地区：[1]Department of Chemistry and Institute of Biomedical Sciences, Fudan University, Shanghai 200433, China

出　　处：《Science China Chemistry》2011年第8期1327-1333,共7页中国科学（化学英文版）

基　　金：supported by the National Basic Research Program of Chi-na (2007CB714506);the National Natural Science Foundation of China (20735005 & 20925517);Science and Technology Committee of Shanghai (10XD1406000 & 09JC1402600);Shanghai Leading Academic Disci-pline B109

摘　　要：Protein phosphorylation as one of the most important post-translational modifications in mammalian cells regulates numerous biological processes. Here we propose a novel strategy for the selective isolation and sensitive analysis of mul- ti-phosphopeptides based on TiO2-gratfed mesoporous materials, in which MCM-41 and SBA-15 were chosen as the hard templates. The commercialized IMAC and TiO2 nanopartices were further investigated in the phosphopeptide analysis for comparison. The enrichment efficiency was evaluated and measured by MALDI-TOF mass spectrometry. The results indicated that both TiO2-SBA-15 and TiO2-MCM-41 exhibited the preferential affinity to multi-phosphopeptides compared with the other two widely used strategies. The mesoporous TiO2 based protocol showed highly selective and sensitive properties, where phosphopepddes could be identified at femtomole.Protein phosphorylation as one of the most important post-translational modifications in mammalian cells regulates numerous biological processes. Here we propose a novel strategy for the selective isolation and sensitive analysis of multi-phosphopeptides based on TiO2-gratfed mesoporous materials, in which MCM-41 and SBA-15 were chosen as the hard templates. The commercialized IMAC and TiO2 nanopartices were further investigated in the phosphopeptide analysis for comparison. The enrichment efficiency was evaluated and measured by MALDI-TOF mass spectrometry. The results indicated that both TiO2-SBA-15 and TiO2-MCM-41 exhibited the preferential affinity to multi-phosphopeptides compared with the other two widely used strategies. The mesoporous TiO2 based protocol showed highly selective and sensitive properties, where phosphopeptides could be identified at femtomole.

关 键 词：TIO2 mesoporous materials multi-phosphopeptides ENRICHMENT 

分 类 号：TQ134.11[化学工程—无机化工] TB383[一般工业技术—材料科学与工程]