Adenovirus-mediated human brain-derived neurotrophic factor gene-modified bone marrow mesenchymal stem cell transplantation for spinal cord injury  

作　　者：ChangshengWang Jianhua Lin Chaoyang Wu Rongsheng Chen 

机构地区：[1]Department of Spinal Surgery, First Affiliated Hospital of Fujian Medical University, Fuzhou 350005, Fujian Province, China

出　　处：《Neural Regeneration Research》2011年第16期1211-1216,共6页中国神经再生研究（英文版）

基　　金：Research Fund for the Doctoral Program of Higher Education of China, No. 20060392003

摘　　要：Rat bone marrow mesenchymal stem cells expressing brain-derived neurotrophic factor were successfully obtained using a gene transfection method, then intravenously transplanted into rats with spinal cord injury. At 1,3, and 5 weeks after transplantation, the expression of brain-derived neurotrophic factor and neurofilament-200 was upregulated in the injured spinal cord, spinal cord injury was alleviated, and Basso-Beattie-Bresnahan scores of hindlimb motor function were significantly increased. This evidence suggested that intravenous transplantation of adenovirus- mediated brain-derived neurotrophic factor gene-modified rat bone marrow mesenchymal stem cells could play a dual role, simultaneously providing neural stem cells and neurotrophic factors.Rat bone marrow mesenchymal stem cells expressing brain-derived neurotrophic factor were successfully obtained using a gene transfection method, then intravenously transplanted into rats with spinal cord injury. At 1,3, and 5 weeks after transplantation, the expression of brain-derived neurotrophic factor and neurofilament-200 was upregulated in the injured spinal cord, spinal cord injury was alleviated, and Basso-Beattie-Bresnahan scores of hindlimb motor function were significantly increased. This evidence suggested that intravenous transplantation of adenovirus- mediated brain-derived neurotrophic factor gene-modified rat bone marrow mesenchymal stem cells could play a dual role, simultaneously providing neural stem cells and neurotrophic factors.

关 键 词：brain-derived neurotrophic factor bone marrow mesenchymal stem cells gene modification intravenous transplantation spinal cord injury neural regeneration 

分 类 号：Q421[生物学—神经生物学] Q813[生物学—生理学]