Effects of antigliomatin from the scorpion venom of Buthus martensii Karsch on chloride channels on C6 glioma cells  

作　　者：Zan Wang Mingxian Li Hongmei Meng Min Huang Weihong Lin Li Cui Shao Wang 

机构地区：[1]Department of Neurology, First Hospital, Jifin University, Changchun 130021, Jilin Province, China [2]Department of Respiratory Medicine, First Hospital, Jilin University, Changchun 130021, Jilin Province, China [3]Department of Physiology, School of Basic Medical Sciences, Jifin University, Changchun 130021, Jilin Province, China

出　　处：《Neural Regeneration Research》2011年第18期1365-1369,共5页中国神经再生研究（英文版）

基　　金：the Science and Technology Development Program of Jilin Province, No.20050407-6

摘　　要：Using whole-cell patch-clamp recordings, the effects of antigliomatin were observed on chloride channels on C6 glioma cells cultured in vitro. Antigliomatin was extracted from the venom of the scorpion Buthus martensii Karsch. Chloride channels are closed under normal osmotic pressure. When osmotic pressure was reduced to 120, 110 and 100 mV, the cell volume enlarged, chloride channels opened, and the chloride channel current increased. Three minutes after antigliomatin treatment, the chloride channel current decreased in a dose-dependent manner. These results show that antigliomatin extracted from the venom of the scorpion Buthus martensii Karsch diminishes chloride channel currents on C6 glioma cells.Using whole-cell patch-clamp recordings, the effects of antigliomatin were observed on chloride channels on C6 glioma cells cultured in vitro. Antigliomatin was extracted from the venom of the scorpion Buthus martensii Karsch. Chloride channels are closed under normal osmotic pressure. When osmotic pressure was reduced to 120, 110 and 100 mV, the cell volume enlarged, chloride channels opened, and the chloride channel current increased. Three minutes after antigliomatin treatment, the chloride channel current decreased in a dose-dependent manner. These results show that antigliomatin extracted from the venom of the scorpion Buthus martensii Karsch diminishes chloride channel currents on C6 glioma cells.

关 键 词：ANTIGLIOMATIN C6 glioma cells chloride channels osmotic pressure whole-cell patch-clamp recording 

分 类 号：Q26[生物学—细胞生物学] Q424