黄芪中黄芪多糖含量的测定  

Determination of Astragalus Polysaccharides in Astragalus

作　　者：赵强强[1] 韩丽[1] 潘媛[1] 王淼[1] 杨明[1,2] 

机构地区：[1]成都中医药大学中药材标准化教育部重点实验室,四川成都611137 [2]江西中医学院现代中药制剂教育部重点实验室,江西南昌330004

出　　处：《中国现代中药》2011年第7期29-30,37,共3页Modern Chinese Medicine

基　　金：国家科技重大新药创制专项(2009ZX09103-307)

摘　　要：目的:建立黄芪中黄芪多糖的含量测定方法。方法:采用比色法测定黄芪多糖的含量。结果:测定波长为489nm,葡萄糖在2.0～14.0μg·mL-1线性关系良好,平均加样回收率为99.84%,RSD=3.71%(n=6)。结论:该方法简便、准确、重复性好,可用于黄芪中黄芪多糖的含量测定。Objective： To establish a method for determining astragalus polysaccharides in Astragalus. Methods： Determined the content of astragalus polysaccharides with colorimetry. Results： The wavelength of determination is 489 nm, The linear relationship of glucose was the range of 2.0 ～ 14. 0μg/ mL^-1, The average recovery was 99. 84 % and RSD was 3.71% （ n = 6 ）. Conclusion： The method is convenient, accurate, good reproducibility and suitable for the quality control of astragalus polysaccharides in Astragalus.

关 键 词：黄芪多糖APS 苯酚-硫酸法 含量测定 

分 类 号：R285.5[医药卫生—中药学]