罗布麻DNA导入棉花选育抗虫种质系  

Insect Resistant Stock Selected by Introducing Dogbane DNA into Cotton

作　　者：沈法富[1] 于元杰[1] 张学坤 刘风珍[1] 尹承佾[1] 

机构地区：[1]山东农业大学农学系,泰安271018 [2]山东省棉花研究中心,济南250100

出　　处：《华北农学报》1999年第4期34-38,共5页Acta Agriculturae Boreali-Sinica

摘　　要：鲁棉6 号自花授粉24 h 后,利用微注射技术,将多酚和单宁含量较高的罗布麻DNA 导入其子房中。在高选择压力下,利用自然虫源,选育出了兼抗棉铃虫和棉蚜的棉花种质系115 。对115 和亲本鲁棉6 号进行了室内生物学测定和食物利用试验检测,结果表明,抗虫种质系115 严重阻碍了棉铃虫的食物利用,显著降低了初孵棉铃虫的存活、生长和发育。而鲁棉6 号对棉铃虫影响较小。115 幼叶棉酚和单宁的含量分别比鲁棉6 号增加了90-9 % 和136-7 % 。After self pollination twenty four hours,the exogenous bagbane(A.Venetum L.)DNA was introduced into ovaries of variety lumian 6(G.hirsutum)by injection method.In high selection stress,the second generation was screened for insect resistant by using natural insect.The stock 115 with resistance to bollworm and aphid was selected.Laboratory bioassay and food utilization of bollworm were used to test 115 and lumian 6.The results showed that leaf square and small boll of 115 highly inhibited food utilization of cotton bollworm,and significantly reduced larval surial,growth and development,whereas the effect was small in lumian 6.Contents of gossypol and tani in leaf of 115 were 90.9% and 136.7% as much as that of Lumian 6.

关 键 词：棉花 罗布麻 DNA 转育 抗虫性 棉铃虫 

分 类 号：S562.034[农业科学—作物学]