受乙烯诱导表达的蜻蜓凤梨MAPKK基因的克隆与序列分析  

Cloning and Sequencing of AfMKK1,A Novel MAPKK Gene Induced by Ethylene in Aechmea fasciata

作　　者：张鲲[1,2] 徐立[2] 丛汉卿[1,2] 丁静[1,2] 郝宏刚[1,2] 李志英 

机构地区：[1]海南大学农学院,海南海口570228 [2]中国热带农业科学院热带作物品种资源研究所,海南儋州571737 [3]农业部热带作物种质资源利用重点开放实验室/海南省热带作物种质资源遗传改良与创新重点实验室,海南儋州571737

出　　处：《热带生物学报》2011年第2期107-112,共6页Journal of Tropical Biology

基　　金：海南省自然科学基金项目(808191);中国热带农业科学院博士启动基金项目(Pzsb0806;Rkyy0604);中央级公益性科研院所基本科研业务费专项资金项目(PZS004)

摘　　要：利用抑制性差减杂交文库筛选到的1个被乙烯诱导并与已知植物促丝裂原活化蛋白激酶(mitogen-activated protein kinase kinase,MAPKK;MKK)同源的cDNA片段,通过RACE技术得到该基因的全长cDNA序列。生物信息学分析表明,该基因属于植物MAPKK的A亚族,具有植物MAPKK的磷酸化一致序列S/T*****S/T。利用半定量RT-PCR检测该基因的表达量在乙烯处理后增强,推测其在乙烯诱导途径中起重要作用。A cDNA fragments homologue of the known plant mitogen activated protein kinase（MAPKK,MKK,MEK） was selected by the suppression subtractive hybridization（SSH） library,and which can be induced by ethylene,and Rapid Amplification of cDNA Ends（RACE） were used to obtain the full length cDNA.Bioinformatic analysis results suggested that the gene belongs to a subfamily of plant MAPKK with a conserved region phosphorylation consensus sequence S/T＊＊＊＊＊S/T.Semi-quantitative RT-PCR results indicated that the expression of this gene increased after ethylene treatment,which suggested it may play an important role in the ethylene induction pathway.

关 键 词：乙烯 蜻蜓凤梨 AfMKK1 克隆 

分 类 号：Q78[生物学—分子生物学] S682.39[农业科学—观赏园艺]