天然彩色棉纤维特异表达启动子LTP3的克隆及其在烟草中的表达特异性  

Cloning of Fiber-specific Promoter LTP3 from Color Cotton (Gossyium hirhsutum) and Its Expression Specificity in Tobacco

作　　者：智联腾[1] 赵倩[1] 敖光明[1] 于静娟[1] 

机构地区：[1]中国农业大学生物学院/农业生物技术国家重点实验室,北京100083

出　　处：《热带生物学报》2011年第2期133-137,共5页Journal of Tropical Biology

摘　　要：报道天然彩色棉纤维特异表达启动子LTP3,大小为1 548 bp。经测序分析,与GenBank中登录的海岛棉(Gossypium hirutum L.DES119)特异表达启动子序列同源性为99%,仅有个别碱基发生改变,而启动子的基本元件未发生突变。将克隆的启动子与报告基因GUS融合,构建植物表达载体pBI-LTP3,并转化烟草。结果表明GUS基因仅在转基因烟草叶片表皮毛中表达。PCR were performed to clone LTP3 promoter from color cotton.Sequencing analysis results indicated that it contained 1 548 nt;the homology is 99% compared with the LTP3 reported by Liu,and only 4 nucleotides were changed.The LTP3（1548bp） promoter was fused with the E.coli.β-glucuronidase gene（GUS） and transformed into tobacco.The results suggested that GUS expressions under control of LTP3 promoter were detected only in the trichomes of transgenic tobacco leaves,while the positive control,contains Cauliflower mosaic Virus（CaMV） 35S promoter and GUS gene,was constitutively expressed,and there are no GUS expression in the negative control（untransformation plants）.The results indicated that LTP3 promoter can be used to express the heterologous genes in tobacco leaves.

关 键 词：天然彩色棉花 纤维特异表达 启动子 

分 类 号：S562[农业科学—作物学] Q785[生物学—分子生物学]