热带兰花2种病毒的ELISA检测  

ELISA Detection of Two Viruses in Tropical Orchid

作　　者：宋海超[1] 张勇[2] 邢波 谢玉成 刘福秀[2] 史学群[1] 

机构地区：[1]海南大学环境与植物保护学院,海南海口570228 [2]海南出入境检验检疫局,海南海口570311 [3]三亚市柏盈热带兰花有限公司,海南三亚572029 [4]海口市美兰区科工信局,海南海口570203

出　　处：《热带生物学报》2011年第2期148-152,共5页Journal of Tropical Biology

基　　金：海南省重大科技研发专项(080103);海南省自然科学基金(309138);国家科技富民强县项目之"兰花优良品种选育及无土栽种"标准化技术示范推广

摘　　要：采用三抗夹心酶联免疫吸附法(TAS-ELISA)和双抗夹心酶联免疫吸附法(DAS-ELISA)检测了海南主要兰花种植基地的疑似病毒感染的兰花叶片。结果表明,海南兰花病原病毒主要为建兰花叶病毒(Cym-bidium mosaic virus,CymMV)、齿兰环斑病毒(Odontoglossum ring-spot virus,ORSV),以及二者复合感染;感染建兰花叶病毒的植株比感染齿兰环斑病毒的植株多。该法具有较高的灵敏度、准确性和特异性,可应用于兰花CymMV和ORSV 2种病毒的早期诊断和检测。The TAS-ELISA and DAS-ELISA were established to detect the orchids suspected to be infected by virus mainly from main orchid planting in Hainan Province.The results suggested that the pathogen were Cymbidium mosaic virus（CymMV）,Odontolossum ring spot virus（ORSV） and their mixed infection;there were more orchids which were infected with CymMV than with ORSV.The method possesses high sensitivity,accuracy and specificity.

关 键 词：兰花 建兰花叶病毒 齿兰环斑病毒 检测 

分 类 号：S682.31[农业科学—观赏园艺] R446.6[农业科学—园艺学]