壮骨止痛方药效部位的筛选及其对骨质疏松雌鼠骨代谢相关激素的影响  

Effective Components of Zhuanggu Zhitong Prescription and its Influence on Hormones Related to Bone Metabolism in Female Rats

作　　者：甘国兴[1] 莫新民[1] 李劲平[2] 张国民[1] 范娟娟[1] 刘仕杰[1] 

机构地区：[1]湖南中医药大学,长沙410007 [2]中南大学药学院,长沙410013

出　　处：《中国实验方剂学杂志》2011年第15期126-130,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(30873291);湖南中医药大学中医内科重点学科项目

摘　　要：目的:筛选壮骨止痛方的药效部位,研究其对骨代谢相关激素的影响,探讨其可能的作用机制。方法:选取3月龄250 g左右SD雌性大鼠150只,按体重随机分为模型组、假手术组、正常组、壮骨止痛方无水乙醇提取A部位组、无水乙醇提取B部位组,石油醚提取A部位组,水提A部位组,水提B部位组,乙酸乙酯提取A部位组,壮骨止痛方组,共10组,每组15只。去卵巢术后5 d拆线后开始给药,连续13周。取血和骨标本从骨密度、骨生物力学、病理形态学等几个方面研究其药效,从血清雌激素(E2)、骨钙素(BGP)、甲状旁腺素(PTH)、降钙素(CT)、瘦素、胰岛素方面研究其作用机制。结果:与模型组相比,壮骨止痛方无水乙醇提取A部位、水提B部位、石油醚提A部位能明显增加去卵巢模型鼠股骨、腰椎骨的骨密度(P<0.05或P<0.01);能显著提高股骨头平均骨小梁密度,差异非常明显(P<0.01),能显著增强去势骨质疏松模型大鼠胫骨的最大应力和最大抗弯强度(P<0.05或P<0.01);与壮骨止痛方组比较无明显差异。与去势模型组比较,壮骨止痛方能显著增加血清E2,预防雌激素不足导致的子宫萎缩(P<0.05);显著降低去势模型鼠血清BGP,PTH,CT(P<0.05)。结论:壮骨止痛方无水乙醇提取A部位、水提B部位、石油醚提A部位是壮骨止痛方治疗骨质疏松症的药效部位;壮骨止痛方能够增加去卵巢骨质疏松模型大鼠血清E2含量,降低血清BGP,PTH,CT,直接或间接调节了机体的骨代谢和细胞因子,改善骨基质的分子结构,加强骨生物力学性能,从而治疗骨质疏松症。Objective：To find the effective components of Zhuanggu Zhitong prescription,investigate its influence on hormones related to bone metabolism and to explore its possible mechanism.Method：One hundred and fifty SD female rats weighing around 250 g（three months old） were randomly divided into model group（ovariectomized,OVX）,sham operation group,control group,ethanol extraction group A,ethanol extraction group B,petroleum ether extraction A position group,water extraction site group A,water extraction site group B,Fraction extracted with ethyl acetate group A,Zhuanggu Zhitong prescription group,a total of 10 groups（n=15 each）.Five days after stitches of the operation of taking out ovaries,different treatments were carried out for 13 weeks.Blood and bone samples were collected for investigations on bone mineral density,bone biomechanics,pathology and other aspects to evaluate efficacy,and serum estradiol（E2）,bone gla protein（BGP）,parathyroid hormone（PTH）,calcitionin（CT）,to explore the effective mechanism.Result：Compared with model group,Zhuanggu Zhitong prescription group A part of ethanol extraction,water extraction B parts,petroleum ether A site could significantly increase the levels of OVX rats femur,lumbar spine bone mineral density（P〈0.05,P〈0.01）;could increase osteoporosis in ovariectomized rat tibia model the maximum stress and maximum bending strength（P〈0.05,P〈0.01）;could significantly increase the average trabecular bone density of the femoral head,the difference was significant（P〈0.01）,with no significant difference to Zhuanggu Zhitong prescription group.Compared to OVX model group,Zhuanggu Zhitong prescription could only significantly increase in serum E2,prevent uterine atrophy caused by insufficient estrogen（P〈0.05）;Zhuanggu Zhitong prescription could significantly decrease serum BGP,PTH,CT for the castrated rats（P〈0.05）.Conclusion：The ethanol extract parts of group A,water extraction site group B,petroleum ether extract A position grou

关 键 词：骨质疏松 壮骨止痛方 药效部位 

分 类 号：R285.5[医药卫生—中药学]