芪附汤抑制阿霉素心脏毒性损伤大鼠心肌细胞凋亡的研究  

Effect of Qifu Decoction on Adriamycin-induced Cardiac Apoptosis in Rat

作　　者：于彩娜[1] 范颖[1] 林庶茹[2] 

机构地区：[1]辽宁中医药大学方剂学科,沈阳110032 [2]辽宁中医药大学中医分子生物实验室,沈阳110032

出　　处：《中国实验方剂学杂志》2011年第15期148-151,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：辽宁省教育厅科研创新项目(2007T117)

摘　　要：目的:研究芪附汤对阿霉素(adriamycin,ADR)引起的心肌细胞凋亡的影响并探讨其作用机制。方法:SD大鼠56只,雄性,随机分为正常组、模型组、中药对照组、芪附汤组,每组各14只。正常组:不予特殊处理。模型组:每周1次ip ADR 2.5mg.kg-1,正常组与模型组分别于造模同时按10 mL.kg-1剂量ig蒸馏水。中药对照组:于造模同时按3.5 mL.kg-1剂量ig生脉饮。芪附汤组:于造模的同时按2.625 g.kg-1剂量ig芪附汤水煎液。每日1次,连续4周后称取大鼠体质量。处死大鼠,称取心脏质量,计算心脏指数(心脏质量/体质量),采用细胞流式检测术测定心肌细胞凋亡率,实时定量PCR法检测Bax,Bcl-2和Caspase-3的mRNA表达水平,计算Bcl-2/Bax。结果:与正常组相比,ADR可使大鼠心脏质量下降,且显著引起大鼠心肌细胞凋亡;Bax和Caspase-3的mRNA表达水平在ADR给药后明显上调,Bcl-2 mRNA表达水平则下调(P<0.05);而芪附汤抑制了Bax,Caspase-3的高表达,同时使Bcl-2 mRNA的表达水平上调,进而使Bcl-2/Bax显著增高(P<0.05)。结论:芪附汤可抑制ADR引起的心肌细胞凋亡,其作用机制与抑制Bax,Caspase-3的mRNA高表达和上调Bcl-2 mRNA表达水平有关。Objective：To investigate the effect of Qifu decoction on adriamycin-induced cardiac apoptosis in rat and reveal possible mechanisms.Method：Fifty-sixe male Sprague Dawley（SD） rats were randomly divided into four groups（the normal group,the model group,Chinese medicine control group and Qifu decoction group with 14 rats in each group）.The normal group：no special treatment,the model group：the rats were treated with ADR 2.5 mg·kg^-1,once per week,ip,in the meantime,the rats of the normal group and the model group were given distilled water,10 mL·kg^-1,ig.Chinese medicine group：pulse-activating decoction was administrated at the same time of modeling,3.5 mL·kg^-1·d-1,ig.Qifu decoction group：Qifu decoction was administrated at the same time of modeling,2.625 g·kg^-1·d-1,ig.After 4 weeks,the rats’ body weight were weighed,and then the rats were executed and their heart were weighed,to calculate cardiac index（heart weight /body weight）,Cardiac apoptosis rate was measured by flow cytometry.mRNA expression level of Caspase-3,Bcl-2 and Bax were detected by real time polymerase chain reaction（Realtime quantitative PCR）,to value the radio of Bcl-2/Bax.Result：Adriamycin administration greatly decreased heart weight and caused remarkable cardiac apoptosis.Elevated mRNA expression of Bax and Caspase-3 were obviously observed after Adriamycin treatment,while the elevation was prevented by Qifu decoction.Adriamycin administration reduced the expression level of Bcl-2 mRNA,while Qifu decoction enhanced it.so Bcl-2/Bax ratio was increased by Qifu decoction,all with statistical significance（P〈0.05）.Conclusion：Qifu decoction could prevent cardiac apoptosis induced by adriamycin,which might be mediated through the prevention of Bax and Caspase-3 mRNA upregulation and Bcl-2 mRNA downregulation.

关 键 词：芪附汤 阿霉素 心肌细胞凋亡 

分 类 号：R285.5[医药卫生—中药学]