黄芪多糖对大鼠胰岛素敏感性的影响  

Effects of Astragalus Polysaccharides on the Insulin Resistance in Rats

作　　者：魏学娟[1] 陈雪辉[1] 翁孝刚[1] 王涛[1] 王缭[2] 

机构地区：[1]新乡医学院第一附属医院内分泌科,河南卫辉453100 [2]南阳医学高等专科学校第三附属医院,河南南阳473000

出　　处：《中国实验方剂学杂志》2011年第15期156-160,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：河南省科技厅资助项目(224630170)

摘　　要：目的:探讨黄芪多糖(APS)对高脂饲料诱导的大鼠胰岛素抵抗(IR)的治疗作用及机制。方法:将48只成年雄性SD大鼠随机分为正常对照组(NC组,12只)和高脂模型组(HFM组,36只),分别给予普通饲料和高脂饲料喂养。模型建立成功后,再次随机分组:高脂对照组(HFC组)、APS组和吡格列酮组(Pio组),每组均12只,分别给予生理盐水,200 mg.kg-1.d-1APS,20 mg.kg-1.d-1 Pio干预8周。随机选取各分组中的一半大鼠进行高胰岛素-正葡萄糖钳夹试验,以葡萄糖输注率(GIR)判定机体的胰岛素敏感性。同时测定另一半大鼠的空腹血糖(FBG),空腹胰岛素(FINS),游离脂肪酸(FFA),脂联素(APN)和抵抗素水平。结果:与NC组相比,HFC组大鼠血浆的FINS,FFA和抵抗素水平升高,GIR和APN水平降低(P<0.05);与HFC组相比,APS组大鼠血浆的FINS,FFA和抵抗素水平降低,APN水平和GIR升高(P<0.05)。GIR和血浆FINS、抵抗素、FFA浓度呈负相关,和血浆APN水平呈正相关(P<0.05)。结论:APS可以改善大鼠的胰岛素抵抗,可能与其升高血浆APN水平和降低血浆抵抗素有关。Objective：To study the mechanisms of ameliorating insulin resistance（IR） by astragalus polysaccharides（APS） in rats with IR.Method：Fourty-eight healthy male Sprague-Dawley rats were randomly divided into two groups：the normal control group（NC group,n=12） and the high fat-diet-induced model group of IR（HFM group,n=36）.Rats in the NC group were fed with ordinary diet while those in the HFM group were fed with high fat diet for six weeks.Then the rats in the HFM group were randomly divided into three groups：the high fat-diet-induced control group（HFC group,n=12）,pioglitazone group（Pio group,n=12）,APS group（n=12）,lavaged with saline,pioglitazone（20 mg·kg^-1·d^-1） and APS（200 mg·kg^-1·d^-1） respectively for eight weeks.Changes in fasting blood glucose（FBG）,fasting insulin（FINS）,free fat acid（FFA）,resistin and adiponectin of rats were routinely measured,meanwhile glucose infussion rate of tissue was evaluated by hyperinsulinaemic-euglycaemic clamp technique.Result：The levels of plasma FINS,FFA,resistin of rats were significantly higher and the levels of APN,glucose inffusion rate of tissues were significantly lower in the HFC group than those in the NC group（P〈0.05）.Compared with those in the HFC group,the above mentioned indexes in APS group were improved significantly（P〈0.05）.GIR and the levels of FFA,FINS,resistin,APN were conducted linear correlation analysis and showed significant correlations.Conclusion：The results indicate that APS can regulate part of the insulin signaling in IR serum,and that APS could be a potential insulin sensitizer for the treatment of IR by an increase of adiponectin and reduction of resistin in serum.

关 键 词：黄芪多糖 高胰岛素-正葡萄糖钳夹技术 胰岛素抵抗 脂联素 抵抗素 

分 类 号：R285.5[医药卫生—中药学]