正交模拟法研究护骨胶囊各单味药及复方药效  

Pharmacodynamic Contributed Values of Composition and Pharmacodynamic Simulation of Compound Hugu Capsules by Orthogonal Simulation Method

作　　者：贾欢欢[1] 高建林[1] 陈珺[1] 卢丽[1] 陆幸妍[1] 杨国柱[1] 曾昭利 曾雯 秦中华 李英彬[1] 万超[3] 李青南[1] 

机构地区：[1]广东药学院,生命科学与生物制药学院,新药功能研究中心,广州510006 [2]东莞万成制药有限公司,广东东莞523040 [3]香港中文大学医学院,生物医学学院,干细胞与再生医学中心,中国香港

出　　处：《中国实验方剂学杂志》2011年第15期161-165,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：广东省教育部产学研结合项目(2009B090300024)

摘　　要：目的:应用正交模拟法分析护骨胶囊(HG)各药味对该复方药效的贡献值,并对该方不同组合的复方进行药效模拟。方法:采用正交模拟法,将HG 10个药味按照设计的正交表分为13个组(第13组为阴性对照组),通过血清药理学实验得出相应含药血清,采用CCK-8法检测各组血清对成骨细胞(Ob)增殖的影响。DAS3.0软件处理数据,分析各药味的药效贡献值并对复方药效进行模拟。结果:以Ob增殖为指标,经正交模拟法分析,确定HG各药味在复方中的药效贡献值顺序为:淫羊藿>骨碎补>巴戟天>山药>龟甲>当归>熟地黄>续断>杜仲>制首乌;以Ob增殖为指标,对复方药效进行模拟研究,结果显示27个模拟组方预测药效值明显优于阴性对照组,而且原方最好,4个模拟组方接近于原方。结论:正交模拟法分析HG,确定了HG各药味在复方中的药效贡献值,并发现多个组方的模拟药效与原方接近。Objective：To analysis pharmacodynamic contributed values of composition and pharmacodynamic simulation of compound Hugu capsules（HG）.Method：Ten components of HG were divided into 13 groups（group 13 for negative control group） by orthogonal simulation method,the drug serum was gotten by the serum pharmacological experiments and CCK-8 method was adopted to detect osteoblast（Ob） proliferation.Pharmacodynamic contributed values and pharmacodynamic simulation of compound were analysis by DAS3.0 software.Result：The proliferation of Ob as a index,a sequence of pharmacodynamic contributed values were found by orthogonal simulation method：Epimedii Folium〉Drynariae Rhizoma〉Morindae Officinalis Radix〉Dioscoreae Rhizoma〉Testudinis Carapax et Plastrum〉Angelica Sinensis Radix〉Rehmanniae Radix Praeparata〉Dipsaci Radix〉Eucommiae Cortex〉Polygoni Multiflori Radix Praeparata Multiflorum F.With Ob proliferation as the index,and the results of pharmacodynamic simulation of compound showed that 27 compounds were better than the negative control group,and the original compound is the best in effect,the other four compounds are close to the original compound.Conclusion：Orthogonal simulation method is a method to analyze the Chinese herbal compound.This approach can determine pharmacodynamic contributed values of composition,and pharmacodynamic simulation of compound for HG.The method can provide a lot of information for studying Chinese herbal compound,and is benefit for developing new drug.

关 键 词：正交模拟法 护骨胶囊 成骨细胞 药效贡献值 模拟药效 

分 类 号：R285.5[医药卫生—中药学]