黄连对小鼠焦虑行为影响的研究  

Influence of Coptidis Rhizoma on Anxiety Behavior in Mice

作　　者：郭花玲[1] 崔瑛[1] 李阳阳[1] 李二平[1] 韩强[1] 朱杨杨[1] 

机构地区：[1]河南中医学院,郑州450008

出　　处：《中国实验方剂学杂志》2011年第15期169-172,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：河南省郑州市科技攻关项目(109TGS485-2)

摘　　要：目的:探讨黄连对小鼠的抗焦虑作用及其机制。方法:将雄性小鼠随机分成5组(n=12),分别为模型对照组,阳性对照组,黄连水煎液高、中、低剂量组(1.8,0.9,0.45 g.kg-1),模型组ig生理盐水,阳性组ig地西泮2.5 mg.kg-1,连续ig给药5 d。末次给药0.5 h后,通过高架十字迷宫和明暗箱实验装置,观察黄连对小鼠焦虑的干预作用。用高效液相-荧光检测法测定脑内γ-氨基丁酸(GABA),谷氨酸(Glu),5-羟色胺的含量(5-HT)。结果:黄连水煎液能明显增加小鼠在高架十字迷宫装置上的开臂进入次数和开臂滞留时间,有增加小鼠明暗箱穿箱次数的趋势。不同组别小鼠脑内GABA的含量:黄连组>阳性组>模型组。结论:黄连水煎液有一定的抗焦虑活性。黄连抗焦虑机制与提高小鼠脑内GABA含量有关。Objective：To evaluate the antianxiety effect of Coptidis Rhizoma in mice and study the related mechanism.Method：Male mice were randomly divided into 5 groups（n=12 each,model control group,the positive control group,1.8 g·kg-1 high-dose group Coptidis Rhizoma decoction,the 0.9 g·kg-1 dose,0.45 g·kg-1 low-dose group）.Model group was given normal saline,and the positive group was given 2.5 mg·kg-1 diazepam.Mice were treated with drugs for 5 days.We carried out the elevated plus-maze test and light-dark transitions test half one hour after the last drug administration.HPLC-FLD was applied to detect the content of gama-aminobutyric acid（GABA）,glucose（Glu） and 5-hydroxytryptamine（5-HT） in mouse’s brain.Result：Coptidis Rhizoma decoction could significantly increase the percentage of the times entering the open arms and stay duration in the total period.And it could increase the times of passing the box.The content of GABA in Coptidis Rhizoma high dose group was the highest,and GABA in positive group was more than that in the model group.Conclusion：Coptidis Rhizoma displays anti-anxiety effect in the elevated plus-maze test and light-dark transitions test.The mechanism is related with enhancing the content of GABA in the brain.

关 键 词：黄连 抗焦虑 Γ-氨基丁酸 高效液相-荧光检测 

分 类 号：R285.5[医药卫生—中药学]