桂枝茯苓胶囊对大鼠子宫内膜异位症异位内膜组织中MCP-1和ICAM-1 mRNA的影响  

Effects of Guizhi Fuling Capsule on the Expression of MCP-1 and ICAM-1 mRNA of Ectopic Endometrium in Rats with Endometriotics

作　　者：蔡雪婷[1,2] 胡春萍[2] 胡婷婷[3] 王志刚[2] 曹鹏[2] 王旻[1] 

机构地区：[1]中国药科大学,南京210009 [2]江苏省中医药研究院,南京210028 [3]南京市江北人民医院,南京210035

出　　处：《中国实验方剂学杂志》2011年第15期202-205,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：康缘中医药科技创新基金项目(HV0813KY);国家自然科学基金面上项目(30873410)

摘　　要：目的:研究桂枝茯苓胶囊对大鼠子宫内膜异位症(EMs)异位内膜组织中单核细胞趋化蛋白-1(MCP-1),细胞黏附分子-1(ICAM-1)在蛋白水平和转录水平的影响。方法:改进制作大鼠EMs动物模型,ig桂枝茯苓胶囊0.256,1.024 g.kg-1,28 d后取子宫内膜,采用免疫组化法检测内膜组织中MCP-1,ICAM-1的分布和表达;采用RT-PCR检测异位内膜组织中MCP-1,ICAM-1 mRNA的表达。结果:桂枝茯苓胶囊可降低大鼠子宫内膜异位内膜组织中MCP-1,ICAM-1的表达;高、低剂量组可使MCP-1,ICAM-1 mRNA水平显著下降(P<0.01),两剂量之间无显著性差异。结论:桂枝茯苓胶囊可降低EMS异位内膜组织中MCP-1,ICAM-I的表达,降低其mRNA的表达。Objective：To study the effects of Guizhi Fuling Capsule on expression of monocyte chemoattractant protein-1（MCP-1） and inter-cellular adhesion molecule-1（ICAM-1） mRNA of ectopic endometrium in rats with endometriosis.Method：Endometriosis model of rat was created by self-transplant,and then administrated with Guizhi Fuling Capsule（0.256,1.024 g·kg^-1） orally for 28 days.Expression and distribution of MCP-1 and ICAM-1 in ectopic endometrium were observed by immunohistochemistry.The mRNA expression of MCP-1,ICAM-1 were evaluated with RT-PCR.Result：The expression and distribution of MCP-1 and ICAM-1 were significantly decreased after treatment with Guizhi Fuling Capsule.Both dose showed similar effect without significant difference.Conclution：The distribution of MCP-1 and ICAM-1 in ectopic endometrium of endometriosis can be modified by Guizhi Fuling Capsule and the mRNA expression was decreased.

关 键 词：桂枝茯苓胶囊 大鼠子宫内膜异位症 异位内膜 单核细胞趋化蛋白-1 细胞黏附分子-1 

分 类 号：R285.5[医药卫生—中药学]