三七总皂苷对大鼠脑缺血再灌注损伤的神经保护机制研究  

Neuroprotective Role of Panax Notoginseng Saponins on Cerebral Ischemia-reperfusion Injury in Rats

作　　者：唐婧姝[1] 裴清华[2] 

机构地区：[1]北京大学医学部,北京100191 [2]北京中医药大学第三附属医院,北京100029

出　　处：《中国实验方剂学杂志》2011年第15期210-213,共4页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:探讨三七总皂苷对脑缺血再灌注损伤的保护作用及机制。方法:SD大鼠分成假手术组,模型组,三七总皂苷组。模型组和三七总皂苷组采用线栓法建立大鼠大脑中动脉局灶缺血再灌注损伤模型,三七总皂苷组在缺血前15 min和缺血后6 h分别ip三七总皂苷100 mg.g-1,模型组ip等量生理盐水。术后24 h评价神经行为学变化,测定脑梗死体积,脑组织中伊文思蓝含量及白介素-β(IL-1β),肿瘤坏死因子α(TNF-α),白介素-6(IL-6),白介素-8(IL-8)水平。结果:三七总皂苷可有效降低脑缺血再灌注损伤大鼠的脑梗死体积,明显减轻脑缺血区血脑屏障破坏的程度。三七总皂苷组脑组织中细胞因子及伊文思蓝的含量较缺血再灌注组显著降低(P<0.05)。结论:三七总皂苷对脑缺血再灌注损伤有显著的脑保护作用,其作用机制与降低脑缺血再灌注损伤中脑部细胞因子的表达和降低血脑屏障通透性有关。Objective：To investigate the effect of panax notoginseng saponins（PNS） on cerebral ischemia reperfusion injury in rats.Method：SD rats were divided into sham operation group,model group,PNS group.Model group and PNS group were established ischemia-reperfusion injury by using ligation of middle cerebral artery.PNS was given 15 min before ischemia and 6 h after ischemia,respectively,ip PNS 100 mg·kg-1 for rats in PNS group.Rats in model group and sham group were given at the same time ip with normal saline.The neurologic deficit score,brain infarction size and the levels of interleukin-1β（IL-1β）,tumor meerosis factor-α（TNF-α）,interleukin-6（IL-6）,interleukin-8（IL-8） and content of Evans blue in brain tissue were examined.Result：PNS could reduce infarct volume of cerebral ischemia-reperfusion injury in rats,reduce the extent of the damage in blood-brain barrier significantly.The levels of cytokines and content of Evans blue in PNS group were successively decreased as compared with those in model group（P〈0.05）.Conclusion：PNS has a significant brain protection on ischemia-reperfusion injury.PNS has significant effect of reducing the levels of cytokines,PNS can also reduce the permeability of blood-brain barrier.

关 键 词：三七总皂苷 脑缺血再灌注 细胞因子 血脑屏障 

分 类 号：R285.5[医药卫生—中药学]