前列舒通胶囊对慢性前列腺炎大鼠NGF表达的影响  

Expression of Nerve Growth Factor in Rats Model of Chronic Prostatitis Treated with Qianlieshutong Capsules

作　　者：陈瑾歆[1] 李云祥[2] 王安果[2] 张宗平[2] 

机构地区：[1]川北医学院生物化学教研室,四川南充637000 [2]南充市中心医院川北医学院第二临床医学院,四川南充637000

出　　处：《中国实验方剂学杂志》2011年第15期224-226,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：川北医学院校级科研苗圃基金;四川省卫生厅研究项目(080227)

摘　　要：目的:观测前列舒通胶囊对慢性前列腺炎模型大鼠前列腺组织中神经生长因子(NGF)表达的影响。方法:根据Genbank中大鼠NGF和GAPDH基因保守区域序列设计荧光PCR适用的引物和探针,Taqman荧光PCR相对定量方法检测前列舒通(0.84,0.42,0.21 g.kg-1)ig,连续30 d对慢性前列腺炎症大鼠前列腺组织NGF表达的影响。结果:慢性前列腺炎模型大鼠前列腺组织NGF的表达比正常对照组明显增加,前列舒通治疗可降低模型大鼠NGF的表达。结论:前列舒通可能通过降低NGF的表达治疗和改善慢性前列腺炎的症状。Objective：To detect the expression of nerve growth factor （NGF） in glandula prostata of chronic prostatitis（CP） rat model treated with Qianlie Shutong capsule. Method： The specific primers and probes for Taqman real-time PCR was disigned according to the NGF and GAPDH cDNA sequence. The expression level of NGF as measured by relative quantification real-time PCR in rat model treated with Qianlie Shutong capsule 0.84, 0. 42,0.21 g·kg^-1, ig for 30 days. Result： Treatment with Qianlieshutong decreased the expression of NGF in glandula prostata of rat model with CP significantly compared to the control group. Conclusion： The expression levels of NGF was degraded in CP rat model treated with Qianlieshutong.

关 键 词：慢性前列腺炎 前列舒通 神经生长因子 

分 类 号：R285.5[医药卫生—中药学]