百里醌抑制体内外胰腺癌转移作用  

Anti-metastasis effect of thymoquinone on human pancreatic cancer

作　　者：吴志豪[1] 陈兆[2] 沈跃[1] 黄莉莉[1] 蒋平[1] 

机构地区：[1]温州医学院附属第二医院,浙江温州325027 [2]温州医学院附属眼视光医院,浙江温州325000

出　　处：《药学学报》2011年第8期910-914,共5页Acta Pharmaceutica Sinica

基　　金：温州市科技局资助项目(Y20090121)

摘　　要：为探讨百里醌抑制体内外胰腺癌转移的作用及其机制,本研究应用不同浓度百里醌作用人胰腺癌Panc-1细胞后,观察百里醌对体外Panc-1细胞迁移和侵袭的作用;Western blotting检测胰腺癌细胞中NF-κB和MMP-9表达的变化。体内建立起裸鼠胰腺癌原位移植瘤模型,分成对照组和百里醌组,实验结束后观察百里醌对裸鼠胰腺癌转移的抑制作用,同时免疫组织化学法检测裸鼠胰腺肿瘤组织中CD34、NF-κB和MMP-9的阳性表达。结果表明,百里醌可浓度依赖性抑制体外人胰腺癌Panc-1细胞迁移和侵袭,同时可下调人胰腺癌Panc-1细胞中NF-κB和MMP-9的表达;另外,与对照组相比较,百里醌可明显抑制荷瘤裸鼠中胰腺癌转移,并下调CD34、NF-κB和MMP-9在胰腺肿瘤组织中的阳性表达。总之,结果提示百里醌可抑制体内外胰腺癌转移,该作用可能通过下调NF-κB和MMP-9的表达而实现。Recent studies reported that thymoquinone（TQ）,a component derived from the medicinal spice Nigella sativa（also called black cumin）,exhibited inhibitory effects on cell proliferation of many cancer cell lines.This study was performed to investigate the anti-metastatic effect of thymoquinone on the pancreatic cancer in vitro and in vivo.The results showed that thymoquinone suppressed the migration and invasion of Panc-1 cells in a does-dependent manner.To investigate the possible mechanisms involved in these events,Western blotting analysis was performed,and found that thymoquinone significantly down-regulates NF-κB and MMP-9 in Panc-1 cells.In addition,metastatic model simulating human pancreatic cancer was established by orthotropic implantation of histologically intact pancreatic tumor tissue into the pancreatic wall of nude mice.And administration of thymoquinone significantly reduced tumor metastasis compared to untreated control.Furthermore,the expression of NF-κB and MMP-9 in tumor tissues was also suppressed after treatment with thymoquinone.Taken together,the results indicate that thymoquinone exerts anti-metastatic activity on pancreatic cancer both in vitro and in vivo,which may be related to down-regulation of NF-κB and its regulated molecules such as MMP-9 protein.Consequently,these results provide important insights into thymoquinone as an anti-metastatic agent for the treatment of human pancreatic cancer.

关 键 词：胰腺肿瘤 百里醌 转移 

分 类 号：R963[医药卫生—微生物与生化药学]