气阴两虚证心肌缺血模型方证对应的代谢组学表征  

Metabonomic phenotype of "formula corresponding to pattern types" based on "Qi and Yin deficiency pattern" of myocardial ischemia rat model

作　　者：严蓓[1,2] 阿基业[2] 郝海平[2] 王广基[2] 刘林生[2] 查伟斌[2] 张颖[2] 顾胜华[2] 

机构地区：[1]卫生部北京医院药学部临床药理室,北京100730 [2]中国药科大学药物代谢动力学重点实验室,江苏南京210009

出　　处：《药学学报》2011年第8期976-982,共7页Acta Pharmaceutica Sinica

基　　金：国家"重大新药创制"科技重大专项(2009ZX09304-001;2009ZX09502-004);国家自然科学基金资助项目(30630076);江苏省自然科学基金资助项目(BK2008038;BK2008493)

摘　　要：为了探索"方证对应"的科学内涵,本研究以气阴两虚证心肌缺血为模型,以基于气相色谱/飞行时间质谱(GC-TOF/MS)检测技术的代谢组学方法为技术手段,对比分析了生脉注射液、丹参注射液和普萘洛尔的保护作用。数据处理和模式识别后,无论从PLS-DA散点图直观观察还是药物纠正的异常的内源性化合物个数来看,生脉注射液都显示优于其他两种药物的效果,进一步用与心血管和睡眠密切相关的指标内皮素-1验证了代谢组学的结果。最后分析了受到调节的化合物及相关生化代谢通路,推测生脉注射液的作用与调节能量代谢、脂代谢、氨基酸代谢等多条代谢途径有关,这为生脉"方""对应"用于气阴两虚"证"心肌缺血提供了科学的证据。此发现说明代谢组学在中医药研究中具有巨大的潜力,这为传统中医理论的现代化研究提供了新的研究思路与方法。In order to explore the scientific connotation of ＂Fangzhengduiying（formula corresponding to pattern types）＂,＂Qiyinliangxuzheng（Qi and Yin deficiency pattern）＂ of myocardial ischemia rat model and GC-TOF/MS based metabonomic method were used for comparing the effects of Sheng-mai injection,Salvia injection and propranolol in the present study.After data processing and pattern recognition,Sheng-mai injection showed better efficacy than the other two drugs in accordance with not only visual observation from PLS-DA scores plots but also the number of abnormal endogenous compounds restored to the normal level.Further studies showed that Sheng-mai injection could normalize the level of plasma endothelin-1,the index related to cardiovascular diseases and sleep disorders,which verified the results of metabonomics.Finally,the regulated metabolites and related metabolic pathways were analyzed,and it was supposed that the effects of Sheng-mai injection involved in the alternation of energy metabolism,lipid metabolism,amino acids metabolism,and so on.These findings provided scientific evidence to Shengmai ＂Fang＂ used for ＂Qi and Yin deficiency pattern＂ correspondingly,indicating that metabonomics has great potential in traditional Chinese medical research,which provides a novel approach and way to modernization of traditional Chinese medicine.

关 键 词：代谢组学 气相色谱-飞行时间质谱 方证对应 气阴两虚 心肌缺血 生脉注射液 

分 类 号：R969[医药卫生—药理学]