DNA与抗癌药物道诺霉素相互作用的研究  

Study of the Interaction of DNA with Anticancer-drug DRN

作　　者：赵昌后[1] 孙星炎[1] 刘盛辉[1] 徐春[1] 方禹之[1] 

机构地区：[1]华东师范大学化学系,上海200062

出　　处：《华东师范大学学报（自然科学版）》1999年第3期68-71,共4页Journal of East China Normal University(Natural Science)

基　　金：国家自然科学基金

摘　　要：该文采用荧光光谱法并用溴化乙锭（ＥＢ）作为荧光探针研究了道诺霉素（ＤＲＮ） 与ＤＮＡ相互作用的方式。结果表明：ＤＲＮ 的生色团能够嵌入ｄｓ ＤＮＡ 双螺旋结构的碱基对之间，ＤＲＮ 与ＤＮＡ 的主要作用位点是碱基Ｇ、Ｃ；ＤＲＮ 插入ｄｓ ＤＮＡ 碱其对中，不会破坏碱基对中氢键，不会影响ＤＮＡ 的复性；ＤＮＡ 与ｓｓ ＤＮＡThe interacion of anticancer-drug daunomycin (DRN) with DNA was studied by fluorescence spectroscopy. The results demonstrated flrorescence quenching was significantly observed in the presence of dsDNA, but ssDNA had slightly effect on the daunomycin fluorescence. The results show daunomycin interaction with DNA by intercalation of the chromophore between base pairs. The main interacion sites are G and C. When DRN intercalates into DNA, it does not disrupt the hydrogen bonds between bases of DNA andeffect on hybridization of DNA.

关 键 词：相互作用 道诺霉素 DNA 抗癌药物 

分 类 号：R979.1[医药卫生—药品]