微量热法测定粉防己碱金属配合物的抑菌性  

Bacteriostatic Activities of Metal-Tet Complexes Studied by Microcalorimetry

作　　者：李晶[1] 毕红艳[1] 杜保同[1] 孙艳芝[1] 张洪林[1] 

机构地区：[1]曲阜师范大学化学与化工学院,曲阜273165

出　　处：《应用化学》2011年第8期897-900,共4页Chinese Journal of Applied Chemistry

摘　　要：从中药粉防己中提取粉防己碱。在无水乙醇中粉防己碱分别与M(NO3)2.3H2O(M=Cu,Zn,Co,Ni)反应,合成了4种新的金属配合物,其结构分别用FT-IR和UV等测试技术进行了表征。应用微量量热仪分别测定了不同浓度的粉防己碱及4种粉防己碱的金属配合物、M(NO3)2.3H2O(M=Cu,Zn,Co,Ni)对大肠杆菌代谢作用的热功率-时间曲线,运用Logistic方程计算出细菌的生长速率常数,建立了生长速率常数与药物浓度间的关系,进而确定了最佳抑菌浓度。结果表明,4种金属配合物的抑菌作用均比M(NO3)2.3H2O和粉防己碱强。Four new metal complexes were synthesized by the reaction of tetrandrine （Tet, extracted from Stephania tetrandra S. Moore） with M（NO3）2 ·3H2O（ g = Cu,Zn,Co,Ni） in ethanol and characterized by FT-IR and UV. The power-time curves of baeteriostatic activities of Tet, its four metal complexes and metal nitrates with different concentrations （c） were determined by the titration microcalorimetry method. The growth rate constants （μ） of E. coli were calculated using logistic equation. The relationships of μ-c for the investigated systems were established and the inhibitory concentrations were optimized. By comparing their optimized inhibitory concentrations, it was found that the bacteriostatic activities of the four metal-Tet complexes on E. coli were superior to those of M（ NO3 ）2 ·3H2O and Tet.

关 键 词：粉防己碱 金属配合物 微量量热法 大肠杆菌 最佳抑菌浓度 

分 类 号：O642.3[理学—物理化学]