烟草花叶病毒在受限空间下的自组装行为  

Confined Evaporative Self-assembly of Tobacco Mosaic Virus

作　　者：肖桂花[1] 彭波[1] 王倩[2] 苏朝晖[1] 林园[1,2] 

机构地区：[1]中国科学院长春应用化学研究所,高分子物理与化学国家重点实验室,长春130022 [2]美国南卡罗莱纳大学化学与生物化学系,美国南卡罗莱纳州29208

出　　处：《应用化学》2011年第8期901-906,共6页Chinese Journal of Applied Chemistry

基　　金：基金委创新群体(50621302);美国NSF CHE-0748690,DoD-W911 NF-09-1-236和DOE-BES资助项目

摘　　要：以烟草花叶病毒(TMV)为基本构建单元,研究了其在毛细管、载玻片-载玻片和柱透镜-硅片3种不同受限空间下的自组装行为。结果表明,由于接触线有规律、重复性的粘滑运动,在3种受限体系下TMV均可组装得到高度规整的条带结构。在毛细管内组装可得到垂直于管的长轴方向的有序条带。随接触线深入管中心,条带跨距(l)与带宽(w)均逐渐增大。在2个平行载玻片所构成的可控空间内所得到的条带以2条互相垂直的中心线为轴对称排列,并沿从载玻片边缘至轴向中心方向条带跨距和带宽梯度增大。同样,利用柱透镜-硅片所组成的体系可得到类似平行线状条带,其跨距、带宽及条带高度沿从外部边缘至柱透镜/硅片接触中心方向逐渐减小。在3种体系下,棒状TMV粒子均平行于接触线的方向取向。TMV浓度对组装结构具有显著影响,随着浓度的增加,条带跨距和带宽均增加,直至条带结构消失形成连续膜。Self-assembly of tobacco mosaic virus （TMV） particles by drying TMV buffered solution constrained in a capillary tube, between two parallel glass slides and in a cylinder-on-flat geometry were studied, respectively. Optical microscopy（OM） and atomic force microscopy（AFM） were employed to characterize the patterns produced. Effect of the concentration of TMV on patterns was also explored. The results show that highly ordered structures could be fabricated under these three confined conditions which were attributed to the periodical, repetitive ＂ stick-slip＂ motion of the contact line. Stripes parallel to the contact line but perpendicular to the long axis of the capillary tube were formed in a glass capillary tube after evaporation. As the solution front moved inward, both l（ span of a stripe） and w（width of a stripe） gradually increased. When drying TMV buffered solution trapped between two glass slides, patterns in a tetramerous radial symmetry were produced. From each side of the slide, both l and w increased as stripes approached the longitudinal axis to the center. Parallel gradient straight stripes were obtained when TMV buffered solution was allowed to evaporate from a cylinder-on-flat geometry. As the contact line propagated from the capillary edge toward the cylinder/Si contact center, both 1 and w decreased. The values of l for the outermost, middle and innermost regions are 28.4, 20.9 and 8.4 μm, respectively. While the values of w are 17.5, 9.2 and 0.8μm, correspondingly. Moreover, the height of the stripe decreased from 33.9 nm to 30. 4 nm, finally to 29.5 nm. In all patterns formed under these three kinds of confinement conditions, individual TMV particles aligned parallel to the contact line, which could not only relieve the strong dipole-dipole repulsion between TMV particles, but also effectively minimize the interfacial energy. Concentration of TMV had the same effect on patterns under these three confinements：both l and w increased with the increase of concentration u

关 键 词：受限蒸发 烟草花叶病毒 自组装 

分 类 号：O636.1[理学—高分子化学]