内质网蛋白44(ERp44)通过1,4,5-三磷酸肌醇受体介导基因转录(英文)  

ERp44 Mediates Gene Transcription via Inositol1,4,5-Trisphosphate Receptors in HeLa Cells

作　　者：潘聪燕[1] 周荣斌[2] 陈政 陈颖骁[1] 吴艳云[1] 苗林[1] 殷文璇[1] 姬广聚[1] 

机构地区：[1]中国科学院生物物理研究所生物大分子国家重点实验室,北京100101 [2]北京军区总医院急诊科,北京100700 [3]Molecular&Integrative Physiology,University of Michigayt,MI 48109-5622,[phone],USA

出　　处：《生物化学与生物物理进展》2011年第8期706-712,共7页Progress In Biochemistry and Biophysics

基　　金：supported by a grant from National Basic Research Program of China(2011CB8091004,2009CB918701)~~

摘　　要：细胞核内钙离子浓度的增加可以引起包括钙离子激活的基因转录在内的很多生理功能.运用Western blot、免疫荧光、实时定量聚合酶链反应、钙成像以及外源三磷酸腺苷刺激细胞释放钙离子等试验方法,发现1,4,5-三磷酸肌醇受体和内质网蛋白44(ERp44)在内质网和核膜上都有很好的共定位.外源三磷酸腺苷可以通过1,4,5-三磷酸肌醇受体刺激核内钙瞬变并磷酸化环磷酸腺苷反应原件结合蛋白(CREB)、刺激原癌基因c-Myc的表达.但是,这些功能都能被1,4,5-三磷酸肌醇受体抑制剂2-氨乙氧基二苯酯硼酸(2-APB)和过表达内质网蛋白44(ERp44)所抑制.这些结果均提示在子宫颈癌HeLa细胞中内质网蛋白44(ERp44)通过1,4,5-三磷酸肌醇受体而介导基因转录.Increase in nuclear calcium concentration has several biological effects which include controlling calcium-activated gene transcription.Using extracellular ATP to induce intracellular calcium transient as a model,Western blotting,immunofluorescence,real-time PCR as well as Ca2＋ image studies were carried out.It was found that inositol 1,4,5-trisphosphate receptors（IP3Rs） and endoplasmic reticulum protein 44（ERp44） co-localized on the nuclear envelope and endoplasmic reticulum（ER）.Extracellular ATP induced nuclear calcium transient via IP3Rs and subsequently increased the cAMP response element binding protein（CREB） phosphorylation and the expression of c-Myc.However,all these were inhibited by 2-aminoethoxydiphenyl borate（2-APB）,an IP3Rs inhibitor,and by over-expression of ERp44.These results suggest that ERp44 inhibits gene transcription via IP3Rs in HeLa cells.

关 键 词：内质网蛋白44(ERp44) 三磷酸腺苷 1 4 5-三磷酸肌醇受体 钙瞬变 基因转录 

分 类 号：Q291[生物学—细胞生物学]