流式细胞血小板交叉配型试验方法的建立  

Establishment of the experiment method for flow cytometry of platelet cross match

作　　者：王嘉励[1] 邓晶[1] 徐秀章[1] 丁浩强[1] 陈扬凯[1] 邵媛[1] 叶欣[1] 

机构地区：[1]广州血液中心输血研究所,广东广州510095

出　　处：《中国输血杂志》2011年第6期478-481,共4页Chinese Journal of Blood Transfusion

基　　金：广东省医学科研基金(A2008578;A2009569);广州市医药卫生科技项目(2007-YB-135;2008-YB-136)

摘　　要：目的建立检测灵敏度更高的流式细胞血小板交叉配型(Flow-XM)的试验方法。方法对104名临床血小板输注>3次的患者作Flow-XM试验,同时采用经典补体依赖微量淋巴细胞毒交叉配型(NIH-CDC)方法作对照,比较2种方法的试验结果。结果血小板阳性检出率,Flow-XM试验为60.58%(63/104),NIH-CDC试验为26.92%(28/104)(P<0.01)。结论 Flow-XM试验具有较高的灵敏度,能够对原始结果全程质控,可使血小板交叉配型技术实现了标准化。Objective To set up a more sensitive detection method of platelet cross match using flow-cytomerty.Methods Blood samples from 104 patients who had received platelet transfusion more than three times underwent Flow-XM and NIH-CDC detection.The results between two different techniques were compared.Results In 104 patients,63 were positive in Flow-XM（60.58%）,28 were positive in NIH-CDC（26.92%）,there was significant difference between two methods（P0.01） Conclusion Flow-XM is more sensitive than NIH-CDC,additionally,the computer printouts represent a permanent record of the cross match for retrospective review.Flow-XM may become the standard cross match method as a possible alternative to be recommended.

关 键 词：交叉配型 相关抗体 血小板 流式细胞术 HLA抗体 灵敏度 输注无效 血小板 

分 类 号：R457.11[医药卫生—治疗学] R331.143[医药卫生—临床医学]