A型流感病毒持续感染细胞系来源的IVpi-189病毒生物学性状的初步研究  

Study of Biological Characteristics of the IVpi-189 Virus Derived from Persistent Influenza A Virus-infected Cell Line

作　　者：刘静[1] 张雷英[1] 那立新[1] 闫建忠[1] 刘北星[1] 

机构地区：[1]中国医科大学基础医学院免疫学教研室,沈阳110001

出　　处：《病毒学报》2011年第4期326-330,共5页Chinese Journal of Virology

基　　金：沈阳市科技计划支持项目(项目编号:F10-205-1-34)

摘　　要：为明确E61-24-P15 A型重组流感病毒的第189代传代子病毒(IVpi-189)是否具备流感病毒温度敏感减毒活疫苗候选株的特点,将IVpi-189病毒感染MDCK细胞,并于不同培养温度条件下培养,观察其致细胞病变效应,病毒合成、释放情况,以及不同温度条件下病毒存活时间。结果显示32℃培养温度下,IVpi-189病毒具有等同于亲代野生病毒株的诱导细胞病变能力,而当培养温度上调至38℃,IVpi-189病毒致细胞病变效果出现缓慢且程度明显减轻。空斑形成单位实验发现IVpi-189病毒在38℃培养条件下增殖能力明显下降,其原因与病毒灭活速度及子病毒释放无关,但与感染细胞病毒合成能力下降有关。上述实验结果初步证实流感病毒持续感染细胞系来源的IVpi-189病毒具有温度敏感减毒活疫苗的生物学特性,在许可培养温度条件下具有良好的增殖能力,而在非许可培养温度下,病毒增殖活性受到明显抑制。本研究为流感病毒减毒活疫苗的开发研制提供实验佐证。To investigate biological characteristics of the IVpi-189 progeny virus derived from the culture of influenza A virus as a live-attenuated vaccine candidate.Persistent infection of a cultured cell line with influenza A virus（MDCK-IVpi） was established by incubating continuously influenza virus-infected cells at a lower temperature.The infectious progeny virus derived from MDCK-IVpi cells at the 189rd subculture was designated as the IVpi-189 strain of influenza virus.The cytopathic effect induced by IVpi-189 virus was observed under different temperature conditions.The production of infectious progeny virus was examined at 38 and 32℃ by plaque titration of cell-associated and released virus.IVpi-189 virus showed cytopathic effect as strong as that of IVwt in infected cell line of MDCK at 32℃.However,when culture temperature was raised to 38℃,the cytopathic effect induced by IVpi-189 virus was delayed and less pronounced.Virus growth in IVpi-189 virus-infected cells at 38℃ was significantly reduced as compared with that of IVwt virus,although both viruses yielded nearly equivalent high titers of cell-associated and released virus at 32℃.The reasons of the decreased proliferative ability of IVpi-189 virus at high culture temperature were unrelated with virus inactivation or the release of progeny virus,but associated with the decreased replication of infectious progeny virus in the infected cells.IVpi-189 virus derived from MDCK cells infected persistently with influenza A virus showed biological characteristics as a potential live-attenuated vaccine candidate

关 键 词：持续感染细胞系 IVpi-189病毒 温度敏感减毒活疫苗候选株 

分 类 号：R373.1[医药卫生—病原生物学]