家蚕BmNPV多角体蛋白与外源蛋白共包埋入多角体的研究  

Co-occlusion of Foreign Protein into Polyhedra with BmNPV Polyhedrin

作　　者：相兴伟[1] 杨锐[1] 陈琳[1] 胡小龙[1] 于少芳[1] 吴小锋[1] 

机构地区：[1]浙江大学动物科学学院,杭州310029

出　　处：《病毒学报》2011年第4期366-371,共6页Chinese Journal of Virology

基　　金：教育部博士点基金(20100101110103);浙江省自然科学基金(Y3110058)

摘　　要：为了探索家蚕核型多角体病毒多角体的包装特性,构建了一种不形成多角体但能大量表达绿色荧光蛋白(EGFP)的重组病毒vBmBac(polh-)-5B-EGFP,将其与野生型BmNPV共同感染BmN细胞,于荧光显微镜下观察到EGFP与多角体可以在同一细胞中同时表达。从感染的BmN细胞中收集纯化多角体,观察到多角体能被激发出绿色荧光,进一步利用Western blot证实多角体中含有EGFP。上述结果表明,多角体可以将自身病毒粒子以外的其他病毒粒子的成分包装进入多角体,表明多角体的包装机制中存在非特异性识别机制。In order to make clear the packing mechanism of the BmNPV polyhedra,a polyhedrin gene negative recombinant baculovirus,vBmBac（polh-）-5B-EGFP,expressing EGFP was constructed,and used to infect BmN cells jointly with wild-type BmNPV.Fluorescent microscopic observation demonstrated that EGFP and polyhedrin were expressed simultaneously,and the EGFP expression and polyhedra formation occurred in most of the jointly infected cells.Analysis of the purified polyhedra from jointly infected BmN cells showed that the foreign proteins were present in the polyhedra.The results indicated that BmNPV polyhedrin could incorporate proteins other than viral proteins into the polyhedra.It implies that a nonspecific recognition mechanism exists in the embedment of BmNPV polyhedra.

关 键 词：家蚕核型多角体病毒 多角体蛋白 EGFP 多角体 共同感染 

分 类 号：S884.51[农业科学—特种经济动物饲养]