ICP-AES法测定纳米银在非洲菊切花中的时空分布  

Determination of Nano-Silver Spatiotemporal Distribution in Cut Gerbera Flowers by ICP-AES

作　　者：吕培涛[1] 黄新敏[1] 陆益民[2] 刘季平[1,3] 张昭其[3] 何生根[1] 

机构地区：[1]仲恺农业工程学院生命科学学院,广东广州510225 [2]仲恺农业工程学院化学与化工学院,广东广州510225 [3]华南农业大学园艺学院,广东广州510642

出　　处：《光谱学与光谱分析》2011年第8期2253-2255,共3页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金项目(30771519);广东省自然科学基金重点项目(8251022501000002);广州市重点科技攻关项目(2007Z2-E0071);广州市荔湾区科技计划项目(2008210021)资助

摘　　要：采用电感耦合等离子体发射光谱(ICP-AES)法测定了纳米银在非洲菊(Gerbera hybrida cv.Cross-fire)切花体内的分布与变化特点。分析结果的相对标准偏差为0.14%～2.89%,方法的加标回收率为93.33%～106.67%。该方法简单、快速、可靠、灵敏度高,能满足实际样品分析要求。结果还表明,经5mg.L-1纳米银预处理24h后再瓶插于蒸馏水的非洲菊切花,其茎末端、茎上端和花瓣均有Ag分布,分布规律为茎末端>茎上端>花瓣,显示纳米银可经由花茎基部切口进入非洲菊切花体内并可迁移至各个部位,但主要集中在茎基部下端并发挥持续有效的抗菌作用,进而产生良好的保鲜效果。试验结果对纳米银的测定及其在鲜切花保鲜上的研究与应用具有重要的指导意义。The spatiotemporal distribution of nano-silver in cut gerbera（Gerbera hybrida cv.Crossfire） flowers were determined by inductively coupled plasma-atomic emission spectrometry technique（ICP-AES）.The relative standard deviations of this method were between 0.14% and 2.89%,and the recovery ratio obtained by standard addition method ranged from 93.33% to 106.67%.The method was proved to be simple,rapid,reliable and highly sensitive,which can meet the demands of actua1 sample analysis.The experimental results also showed that Ag could be found in the basal stem end,upper stem end and petal of the cut gerbera flowers treated in nano-silver solution of 5 mg· L-1 for 24 h and thereafter placed in distilled water.However,the Ag content in basal stem ends was much higher than those in upper stem ends and petals.The results indicated that nano-silver particles could enter into the flower stems through the cuts of stem ends and then moved to different parts of the cut gerbera flowers,but most of them located in the basal stem ends during the vase period.The fact that Ag was centred in basal stem end implied that the positive preservation effects of nano-silver on cut gerbera flowers is related to its strong and sustainable antiseptic action in the stem ends of cut flowers.The above results provide a reliable method for the determination of nano-silver and theoretical basis for its futher research and application in the preservation of cut flowers.

关 键 词：ICP-AES 非洲菊 切花 纳米银 时空分布 

分 类 号：O657.3[理学—分析化学] S681.9[理学—化学]