武夷山脉石杉科植物石杉碱甲含量的研究  

Content of Huperzine A in Huperziaceae and Different Populations from Wuyi Mountain

作　　者：张君诚[1,2] 黄晖[1] 张杭颖[1] 钟扬[2] 

机构地区：[1]三明学院应用生物技术研究所,福建三明365004 [2]复旦大学生物多样性与生态工程教育部重点实验室,上海200433

出　　处：《植物遗传资源学报》2011年第4期629-633,共5页Journal of Plant Genetic Resources

基　　金：福建省科技厅重点项目(2010N0027);福建省教育厅科技项目(JA09237)

摘　　要：石杉植物因所含Hup A为高效、可逆、高选择性的中枢性乙酰胆碱酯酶抑制剂而受关注。实地采集标本并用改进的HPLC法测定了武夷山脉4种石杉科植物及不同居群间的石杉碱甲(Hup A)含量。结果显示,石杉碱甲在长柄石杉、四川石杉、有柄马尾杉和华南马尾杉中均有分布,其中以有柄马尾杉中含量最高,达0.151%;含量最低的是四川石杉,为0.016%。该地区优势种长柄石杉(千层塔)11个居群的石杉碱甲含量测定显示:武平县梁野山和三元区中村乡两个居群含量较高,分别为0.0636%和0.0519%。对石松科部分种进行石杉碱甲测定,认为藤石松等不含石杉碱甲。结果表明武夷山脉地区拥有优良的石杉碱甲植物资源。Huperziaceae was recently attracted because of its Huperzine A（Hup A） is a potent,reversible,and selective acetylcholinesterase inhibitor（AChEI）.Hup A in four plants of Huperziaceae from Wuyi mountain were determined by HPLC.The results showed that Hup A were found in all four species（both two genus）,with the highest in Phlegmariurus petiolatus（0.151%）and lowest in Huperzia sutchueniana（0.016%）.Content comparisons of Hup A in Huperzia serrata（Thunb.ex Murray） Trev.var.longipetiolata（spring）H.M.Chang among 11 populations were carried out in this paper,which indicated that Wuping and Sanyuan population had good performance.Hup A in some species near Huperziaceae from this area were also determined.There is no Hup A in Lycopodiastrun casuarinoides.It is also suggested that excellent resources were exist in this area.

关 键 词：石杉碱甲 石杉科 高效液相色谱 居群 武夷山脉 

分 类 号：Q946[生物学—植物学]