复合振动抑制RAW264．7细胞分化成熟  

Compound vibration inhibits RAW264.7 cell differentiation

作　　者：巫松辉[1] 钟招明[1] 查丁胜[1] 陈建庭[1] 

机构地区：[1]南方医科大学南方医院脊柱骨病外,广州510515

出　　处：《中国骨质疏松杂志》2011年第7期564-567,共4页Chinese Journal of Osteoporosis

基　　金：广东省科技计划项目(2008B030301343)

摘　　要：目的研究复合振动对核因子-κB受体活化因子配体(RANKL)诱导的RAW264.7细胞分化的影响,探讨复合振动对破骨细胞分化的影响及机制。方法 RAW264.7细胞RANKL诱导培养3或4d并施加复合振动干预,通过抗酒石酸酸性磷酸酶(TRAP)染色观察TRAP阳性多核细胞形成的变化,real-time RT-PCR分析破骨细胞特异性基因组织蛋白酶K(cathepsin K),金属蛋白酶-9(MMP-9)和TRAP表达的变化。结果复合振动能抑制RANKL诱导破骨细胞形成,下调破骨细胞特异基因cathepsin K,MMP-9和TRAP的表达。结论 RANKL促进RAW264.7细胞向破骨细胞分化,并增加特异基因的表达,但RANKL的促进作用受复合振动抑制。这进一步的阐释复合振动抗骨质疏松的作用机制。Objective To investigate the effect of compound vibration on osteoclast differentiation using receptor activator of nuclear factor-kappa B ligand （RANKL）-treated RAW 264.7 cells, and to explore the mechanism of compound vibration on osteoclast differentiation. Methods RAW264.7 cells in the presence of RANKL were treated with or without compound vibration for 3 or 4 days. Tartrate resistant acid phosphatase （ TRAP） -positive multinuclear cells （MNCs） were examined using TRAP staining. Expression of the osteoclast-speci？ c genes, such as TRAP, cathepsin K, and matrix metallopeptidase-9 （MMP-9）, were analyzed using real-time RT-PCR. Results Compound vibration significantly decreased the number of TRAP-positive MNCs, and down-regulated the mRNA expression of cathepsin K, MMP-9, and TRAP. Conclusions RANKL stimulated osteoclast differentiation by RAW264. 7 cells, and stimulated the expression of osteoclast-specific genes. The RANKL-induced stimulation was inhibited by compound vibration. The finding further explored the anti-osteoporosis mechanism of compound vibration.

关 键 词：RAW264.7细胞 RANKL 破骨细胞 细胞分化 

分 类 号：R454.4[医药卫生—治疗学]