藏红花煎剂对去卵巢大鼠腰椎骨密度及微观结构的影响  

The effect of crocus sativus decoction on bone mineral density and microstructure of the lumbar vertebra in ovariectomized rats

作　　者：曹鹏冲[1] 雷伟[1] 颉强[1] 严亚波[1] 王军[1] 石磊[1] 

机构地区：[1]第四军医大学西京医院骨科研究所,西安710032

出　　处：《中国骨质疏松杂志》2011年第7期574-577,629,共5页Chinese Journal of Osteoporosis

基　　金：863基金项目(2007AA02Z468)

摘　　要：目的研究中药藏红花煎剂对去卵巢大鼠腰椎骨密度及微观结构的影响。方法选用48只4月龄SD雌性大鼠,随机分为6组:假手术组(Sham)、去势组(OVX)、戊酸雌二醇组(E2)、藏红花低剂量组(L-Crocus)、藏红花中剂量组(M-Crocus)、藏红花高剂量组(H-Crocus)。术后4周各组分别给予相应制剂灌胃,术后12周处死,剖取L5椎体标本,采用双能X线吸收骨密度测量仪(DEXA)和显微CT进行测量分析。结果统计分析骨密度(BMD):L-Crocus、M-Crocus、H-Crocus组BMD值均高于OVX组(P<0.01);显微CT结果分析:L-Crocus、M-Crocus、H-Crocus组骨小梁微观结构优于OVX组(P<0.01)。结论藏红花煎剂有助于抑制去卵巢大鼠腰椎骨量的丢失,提高骨密度,改善骨小梁微观结构,对去卵巢大鼠骨质疏松具有防治作用,推测其作用途径可能包括抗氧化、降血脂和抗血凝,具体作用机制尚需进一步实验研究。Objective To study the effect of crocus sativus decoction on the bone mineral density （BMD） and microstructure of the lumbar vertebra in ovariectomized rats. Methods Forty-eight 4-month female Sprague-dawley rats were divided randomly into 6 groups： the sham-operating group （Sham）, the ovariectomized group （ OVX）, estradiol valerate treated group （ E2 ） , low-dose of crocus sativus treated group （L-Crocus）, medium-dose of crocus sativus treated group （M-Crocus）, and high-dose of crocus sativus treated group （H-Crocus）. Rats in each group were administrated with according compounds intragastricly for 4 weeks after surgery. The rats were executed on 12 weeks after surgery. The fifth lumbar vertebras were harvested and analyzed using dual-energy X-ray absorptiometry （DEXA） and micro-CT. Results The BMD analysis showed that BMDs in rats of L-Crocus, M-Crocus, and H-Crocus group were significantly higher than that in rats of OVX group （ P 〈 0.01 ）. The micro-CT reconstruction analysis showed that the microstructure of the bone trabecular in rats of L-Crocus, M-Crocus, and H-Crocus group were significantly better than that in rats of OVX group （P 〈 0.01 ）. Conclusion Crocus sativus was beneficial in the inhibition of bone loss in ovariectomized rats. It could increase BMD and improve the microstructure of bone trabecular, and had preventive effect on osteoporosis in ovariectomized rats. The mechanism of action may include anti-oxidation, blood lipid reduction, anti-coagulation, which needs further study.

关 键 词：藏红花 骨质疏松 骨密度 显微CT 

分 类 号：Q953[生物学—动物学] R68[医药卫生—骨科学]