雷尼酸锶对局部肌麻痹大鼠的骨量丢失及微结构退变的影响  

Effect of strontium ranelate on bone loss and degeneration of bone microachitecture in rats with localized paralysis of the muscle

作　　者：房德玉[1] 张志敏[1] 李爽[1] 张红兵[1] 盛志峰[1] 马育林[2] 廖二元[1] 

机构地区：[1]中南大学湘雅二医院代谢内分泌研究所,长沙410011 [2]中南大学公共卫生学院

出　　处：《中国骨质疏松杂志》2011年第7期578-582,共5页Chinese Journal of Osteoporosis

基　　金：教育部博士点新教师基金项目(20090162120050);湖南省科技厅面上项目(2009RS3014);中国博士后科学基金面上项目(20090461010);中国博士后科学基金特别资助项目(201003512);广东省医学会研究基金(A2009778);广东省中山市科技局资助项目(20091A048)

摘　　要：目的研究雷尼酸锶对局部肌肉麻痹大鼠的骨量丢失及微结构退变的影响。方法 21只3.5月龄的雌性SD大鼠随机分三组,每组各7只:肉毒素组(BTX组),右侧股四头肌肌注肉毒素建立局部肌肉麻痹废用模型;肉毒素+雷尼酸锶组(BTX+SR组),肌注肉毒素并予以雷尼酸锶灌胃处理;对照组。所有大鼠干预9周后处死,取双侧股骨,行显微CT扫描分析。结果 BTX组与对照组相比较,废用侧的股骨皮质骨矿物盐含量、皮质骨骨密度、皮质骨面积和截面总面积以及松质骨的骨体积分数、骨小梁厚度、骨小梁数量和骨小梁联接密度均明显下降;骨小梁面积分数、骨小梁间隔和结构模型指数显著增加(P<0.05)。BTX+SR组与BTX组相比较,皮质骨骨密度、皮质骨面积和截面总面积以及松质骨的组织骨密度、骨体积分数、骨小梁厚度、骨小梁数量和骨小梁联接密度明显增加,骨小梁面积分数、骨小梁间隔和结构模型指数显著下降(P<0.05)。BTX+SR组与BTX组和对照组比较,健侧松质骨的表观骨密度、组织骨密度、骨体积分数和骨小梁厚度均明显增加(P<0.05)。结论雷尼酸锶可阻止因局部肌肉麻痹导致的大鼠骨量丢失和微结构的退变。Objective To evaluate the effect of strontium ranelate （SR） on bone loss and microarchitectural deterioration in rats with localized muscle paralysis. Methods Twenty-one 3.5-month-old female Sprague- Dawley rats were randomly assigned to 3 groups, 7 rats in each group： BTX group contained rats with localized paralysis-disused muscle established by injecting Clostridium botulinum toxin in the right quadriceps femoris; BTX ＋ SR group contained rats received BTX and treated with SR; and a control group. All the rats were sacrificed on 9 weeks after the treatment. Both femurs of each rat were collected for micro- CT analysis Results Compared to those in the control rats, the cortical bone mass, cortical BMD, cortical area, total cross-sectional area, trabecular BV/TV, trabecular thickness （Tr. Th） , trabecular number （Tr. N） , and connectivity density （ Conn. D） in the disused femur significantly decreased, while the trabecular BS/BV, trabecular separation （Tr. Sp） , and structure model index （SMI） significantly increased in the BTX rats （P 〈 0.05 ）. In the BTX rats treated with SR, the cortical BMD, cortical area, total cross- sectional area, trabecular BMD, trabecular BV/TV, Tr. Th, Tr. N, and Conn. D in the disused femur significantly increased, while BS/BV, Tr. Sp, and SMI significantly decreased, compared to those in BTX rats （P 〈0.05）. Compared to BTX group and control group, trabecular BMD, trabecular BV/TV, and Tr. N significantly increased in the intact femur in BTX-SR group （P 〈 0.05）. Conclusion SR could prevent bone loss and microstructural degradation in localized muscle paralysis rats.

关 键 词：雷尼酸锶 废用 骨量丢失 骨微结构 

分 类 号：R589[医药卫生—内分泌]