利用分子标记辅助选育油菜隐性上位互作核不育临保系  

Marker-assisted selection for temporary maintainers of a recessive epistemic genic male sterility in Brassica napus L

作　　者：倪西源[1,2] 徐小栋[2] 黄吉祥[2] 陈飞[2] 周伟军[1] 赵坚义[2] 

机构地区：[1]浙江大学作物科学研究所,浙江杭州310058 [2]浙江省农业科学院作物与核技术利用研究所,浙江杭州310021

出　　处：《浙江大学学报（农业与生命科学版）》2011年第4期407-412,共6页Journal of Zhejiang University:Agriculture and Life Sciences

基　　金：浙江省自然科学基金面上资助项目(Y306440);国家高技术研究发展计划"863"资助项目(2009AA10115);浙江省农业科学院与中国科学院合作资助项目(2007R16Y03E01);浙江省农业科学院重点实验室开放课题资助项目(YS2009ZY206)

摘　　要：以湘油15、沪油15、优88和沪油17分别与临保系HYZ1-TAM杂交,在其F2利用与不育基因Bnms3和隐性上位基因Bnrf连锁的分子标记对临保系基因型单株进行筛选,并对筛选获得的单株进行测交验证确定是否为临保系基因型.结果表明:在4个品种的转育F2群体共355个单株中,利用分子标记辅助选择获得了62个标记基因型为临保系的单株,这些单株经测交证实有57株为真实临保系,分子标记辅助选择的准确率为91.94%.根据实验结果推测,本研究所用的4个甘蓝型油菜品种在Bnms4基因位点未出现显性可育等位基因.实验结果验证了分子标记辅助选择具有较高的准确率,对开展该不育系统的转育工作具有很好的借鉴意义.Four varieties of Brassica napus,Xiangyou 15,Huyou 15,You 88 and Huyou 17,were crossed with a temporary maintainer（TAM） named HYZ1-TAM respectively and produced four F2 populations.In these populations,molecular markers associated with Bnms3 and Bnrf were used to select new temporary maintainer plants which were identified by test-cross.Sixty-two plants with TAM marker-genotype were selected by marker-assisted selection（MAS） from three hundred and fifty-five plants of four F2 populations.Fifty-seven plants（with the accuracy of 91.94%） were TAM genotype proved by test-cross.Compared with traditional method,it is very efficient to select TAM by MAS.In the Bnms4 locus,all the four varieties have a same genotype which is ms4ms4.

关 键 词：甘蓝型油菜 核不育 分子标记辅助选择 临保系 

分 类 号：Q78[生物学—分子生物学] S565.4[农业科学—作物学]