新疆8个地域维吾尔族群线粒体DNAV-区9 bp缺失频率与Y-染色体DYS287位点多态性研究  

Analysis on the Frequency of 9 bp Deletion of mtDNA and on Polymorphisim of Y-choromosome DYS287 Sites in Uighur Population in Eight Geographical Regions of Xinjiang

作　　者：木耶塞尔.伊斯马依力 古丽娜.艾山 马合木提.哈力克 

机构地区：[1]新疆大学生命科学与技术学院资源生物实验室,新疆乌鲁木齐830046

出　　处：《中山大学学报（自然科学版）》2011年第4期100-105,共6页Acta Scientiarum Naturalium Universitatis Sunyatseni

基　　金：国家自然科学基金资助项目(31060152);新疆维吾尔自治区自然科学基金资助项目(2010211A02);新疆大学动物学重点学科资助项目;中国科学院昆明动物研究所合作项目资助项目

摘　　要：为研究新疆8个地域维吾尔族群体的线粒体DNA 9 bp序列缺失频率与Y染色体DYS287位点多态性,分别采用PCR扩增直接测序法和PCR结合琼脂糖凝胶电泳检测法对群线粒体DNA 9 bp缺失频率与Y-染色体DYS287位点多态性进行分析。结果表明在新疆的喀什、和田、库车、且木、哈密、吐鲁番、伊梨和尉梨县的240个无关现代维吾尔族群体中,线粒体DNA 9 bp缺失频率为3.3%,3.3%,6.6%,3.3%,6.6%,3.3%,3.3%,10%。在180个无关现代维吾尔族群男性个体中Y染色体DYS287位点全部显示为YAP-,没有显示为YAP+。结果提示新疆不同地域现代维吾尔族群体的线粒体DNA 9 bp缺失频率相当低,9 bp缺失不是现代维吾尔族的母系遗传传结构特征,而且不同地域维吾尔族群体线粒体DNA 9 bp缺失频率没有显著性的差异。父系遗传结构单一,YAP+不是现代维吾尔族群体的父系遗传特征。研究获得了新疆不同地域维吾尔族群体的线粒体DNA 9 bp序列缺失频率与Y染色体DYS287位点多态性数据,为不同地域维吾尔族群体遗传关系的分析,法医鉴定及不同地域维吾尔族群体之间的起源关系差异提供了一定的遗传背景资料。In order to study the frequency of 9 bp deletion of mtDNA V region and polymorphisim of Y-choromosome DYS287 sites among Uighur population in eight geographical regions of Xinjiang.We used the polymorphism of DYS287 sites in Y choromosome and the defeciency frequency of chromosome DNA 9 bp region by PCR directly sequensing method and by PCR combining with agarose gel electro-phoresis respectively.The results showed： Among 240 unrelated modren Uighur individuals who come from Kashgar,Khotan,Kuqa,and Qarqan,Hulja,Hami,Turpan,and Lopnur in XinJiang,the frequency of 9 bp deletion of mtDNA value as 4%,3.7%,3%,4%,4.5%,4%,3%,4%.Polymorphisim of Y-choromosome DYS 287 sites of 180 modern Uighur male individuals all appears as YAP-,none shows YAP＋.The results suggest that Uighur groups in different parts of Xinjiang show very low frequency in their 9 bp deletion of mtDNA,9 bp deletion is not a modern Uighur matrilineal genetic structure of transfer,Uighurs in different region of Xinjiang share same 9 bp deletion of mtDNA.Paternal genetic structure is simple,YAP ＋ is not a modern Uighur population genetic characteristics of the male line.So,some conclusions can be given：with collecting frequency of 9 bp deletion of mtDNA and polymorphisim of Y-choromosome DYS 287 sites of Uighur population in different regions of Xinjiang,conduct analysis genetic relationship of Uighur population in different region of Xinjiang and Forensic identification provide some background informations about origin relationship Uighur groupsin different regions.

关 键 词：序列缺失 DYS287 多态性 维吾尔族 新疆 

分 类 号：Q343.35[生物学—遗传学]