侵染新疆加工番茄的中国番茄黄化曲叶病毒DNA-A的基因组特征  

Genomic characterization of Tomato yellow leaf curl China virus infecting pro-cessing tomato in Xinjiang

作　　者：都业娟[1] 许文博 向本春[1] 黄家风[1] 

机构地区：[1]石河子大学绿洲农作物病害防控重点实验室,新疆石河子832003 [2]大连市农业科学研究院,大连116036

出　　处：《植物病理学报》2011年第4期393-398,共6页Acta Phytopathologica Sinica

基　　金：国家自然科学基金资助项目(30860162);国家科技部国际合作与交流专项(2008DFA30560)

摘　　要：从新疆加工番茄上分离到病毒分离物XJ26-4,对其基因组DNA-A全序列测定表明,XJ26-4 DNA-A全长2 737个核苷酸（GenBank登录号：FN985163）,具有典型的双生病毒基因组特征。进一步序列比较发现,XJ26-4 DNA-A与中国番茄黄化曲叶病毒（Tomato yellow leaf curl China virus,TYLCCNV）各分离物的同源性最高,达到91.2%~99.5%,而与其他双生病毒的序列相似性均在79.5%以下,表明XJ26-4是TYLCCNV的一个分离物。这是首次明确新疆加工番茄受到粉虱传双生病毒的侵染。The virus isolate XJ26-4 was collected from the leaves of processing tomato showing yellow mo-saic symptom in Xinjiang.The complete nucleotide sequence of DNA-A was determined,it comprised 2 737 nucleotides and had typical genomic organization of begomoviruses.Sequence comparison showed that DNA-A of XJ26-4 had the highest sequence identities（91.2%-99.5%） with that of Tomato yellow leaf curl China virus（TYLCCNV） isolates,while less than 79.5% identities were found when compared with other begomoviruses isolated in Asian and Africa.It was concluded that isolate XJ26-4 infecting processing tomato was one of TYLCCNV isolates.This is the first report of whitefly-transmitted geminviruses in Xinjiang.

关 键 词：加工番茄 中国番茄黄化曲叶病毒 菜豆金色花叶病毒属 DNA-A 

分 类 号：S432.41[农业科学—植物病理学]