应用蛋白宏阵列快速检测西瓜细菌性果斑病菌  

Rapid detection of Acidovorax avenae subsp. citrulli using protein macroarray

作　　者：熊亮斌[1,2] 高丽萍[1] 刘箐 夏俊芳 黄超杰 王天昌 

机构地区：[1]兰州大学,兰州730000 [2]甘肃出入境检验检疫局,兰州730020 [3]兰州慧盟生物科技有限公司,兰州730010

出　　处：《植物病理学报》2011年第4期432-436,共5页Acta Phytopathologica Sinica

基　　金：国家质量监督检验检疫总局项目(2007IK259;2009IK276);甘肃省科技厅中小企业创新基金计划(0802NCCA028)

摘　　要：蛋白阵列（Protein array）也叫蛋白芯片,是生物芯片（Biochip）的一种,主要利用抗原/抗体可特异性结合原理制备而成。按阵列样点大小,蛋白阵列又可分为微阵列（Microarray）和宏阵列（Mac-roarray）两类。Acidovorax avenae subsp.citrulli（Aac） is a seedborne pathogen harmed cucurbitaceae plants se-riously.In this research,a protein macroarray was developed to detect the pathogen rapidly using chemiluminescence.On nitrocellulose membrane,a macroarray was fabricated and it showed satisfactory results in the detectability.The macroarray could detect Aac as low as 2.3×105 cfu/mL without apparently crossing reaction with other seedborne pathogens of cucurbitaceae crops,and it showed a same detectability with ELISA for seed samples.This method also had a good specificity and repeatability.Moreover,60 seed samples were used to test the practicability of this detection method.The results showed that the detection system had a high consistent（60/60） with ELISA assay.This system had identical detectability with only 1/12 capture antibody and 1/8 detection time of ELISA assay and the operation was very convenient.After assembling with a hybri-dization box,it could be generalized to grass roots workstation.Meanwhile,this work provided a basis of simultaneous detection to multiple plant pathogens using protein macroarray.

关 键 词：蛋白芯片 微阵列 快速检测 细菌性 病菌 西瓜 应用 宏 

分 类 号：S435.111.4[农业科学—农业昆虫与害虫防治]