PEG(6000)-Na_2HPO_4双水相体系在鹰嘴豆蛋白萃取中的应用  

Studies on Extraction of Chickpea Protein in(PEG-Na_2HPO_4) Aqueous Two-Phase Systems

作　　者：魏玲[1] 李学琴[1] 孟宪锋[1] 薛来奇[1] 

机构地区：[1]昌吉学院,新疆昌吉831100

出　　处：《食品研究与开发》2011年第7期21-24,共4页Food Research and Development

基　　金：昌吉学院科研基金资助项目(08YJZD001)

摘　　要：对PEG(6000)-Na2HPO4双水相萃取鹰嘴豆蛋白进行研究,考察PEG的浓度、Na2HPO4的量、恒温超声时间、pH、温度等因素对蛋白质萃取率的影响。结果表明:该双水相对鹰嘴豆蛋白的最佳萃取条件:PEG(6000)浓度为10%,PEG与Na2HPO4的质量比为1∶1.5p,H为7.5,温度40℃,在此条件下蛋白质萃取率可达到63.46%。The extraction of chickpea protein in polyethylene glycol（PEG）-（Na2HPO4） aqueous two-phase systems was investigated.The main factors on the extraction rate of chickpea protein were discussed Including the concentration of PEG,the weight of Na2HPO4,the time of ultrasonic,temperature condition,pH.The optimal conditions of extraction for chickpea protein were about PEG（6000）10 %,mass ratio of PEG and Na2HPO4 1∶1.5,pH 7.5,temperature 40 ℃.Finally,the highest extraction rate of protein in above conditions the extraction yield was 63.46 %

关 键 词：PEG(6000)-Na2HPO4双水相 鹰嘴豆蛋白质 萃取 工艺条件 

分 类 号：S529[农业科学—作物学]