高效液相色谱法测定猪肠衣中大环内酯类抗生素残留量的研究  

Determination of Macrolides Antibiotics Residues in Pig Casing by High Performance Liquid Chromatography

作　　者：田苗[1] 林维宣[1] 董伟峰[1] 谢文 于灵[1] 

机构地区：[1]辽宁出入境检验检疫局,辽宁大连116001 [2]浙江出入境检验检疫局,浙江杭州310012

出　　处：《食品研究与开发》2011年第7期117-119,共3页Food Research and Development

摘　　要：建立高效液相色谱法检测猪肠衣中螺旋霉素、替米考星、泰乐菌素、交沙霉素等4种大环内酯类抗生素残留量的方法。试样中用乙腈提取,C18固相萃取柱净化,高效液相色谱测定,外标峰面积法定量。方法的回收率范围为70.2%～94.6%;室内相对标准偏差3.2%～8.9%,4种大环内酯类抗生素的检出限均为0.5 mg/kg。A multiresidue method for the detection of 4 macrolides antibiotics（spiramycin,tilmicosin,tylosin,and josamycin） in pig casing is presented.The method use CH3CN during the extraction procedure and cleanup step over a C18 SPE cartridge.The analytes are separated and detected by high performance liquid chromatography（HPLC） using external standard method.The recoveries for all 4 macrolides antibiotics was 70.2 %-94.6%.The relative standard deviation（RSD） of them was 3.2 %-8.9 %.The method limit of detection were 0.5 mg/kg for all macrolides antibiotics

关 键 词：大环内酯类抗生素 猪肠衣 高效液相色谱 测定 

分 类 号：TS251.7[轻工技术与工程—农产品加工及贮藏工程]