籼稻恢复系空间诱变后代恢复力变异及遗传多态性分析  

Variation of Restoring Ability and Analysis of Genetic Polymorphism in Posterity of Restorer Lines by Space Mutagenesis

作　　者：吴德志[1] 刘永柱[1] 郭涛[1] 张建国[1] 陈志强[1] 王慧[1] 

机构地区：[1]华南农业大学,国家植物航天育种工程技术研究中心,广东广州510642

出　　处：《华南农业大学学报》2011年第3期1-5,共5页Journal of South China Agricultural University

基　　金：863计划项目(2007AA100101);国家科技支撑计划项目(2008BAD97B02)

摘　　要：利用航天搭载处理水稻恢复系R998、桂99和航恢七号种子,从诱变后代中筛选突变体,考察了突变体对不同类型不育系恢复力的变化及其一般配合力,并用水稻SSR引物分析了突变株系的遗传多态性.结果表明:空间诱变能引起籼稻恢复系恢复力的变异;与对照相比,突变体与不育系测交杂种F1的花粉育性表现出一定程度的变化,尤其是出现由恢复系变为保持系的突变体.R998-3、R998-4、桂99-2、桂99-4和航恢七号-4产量相关性状的一般配合力效益值较高.选用分布于12对染色体的104对SSR引物,对比检测原种和突变体基因组间的多态性,共检测了2 538个基因座,基因座多态频率为9.85%,多态性以小片段缺失或插入为主.Mutants were selected from the rice restorer lines of R998,Gui99 and Hanghui No.7 treated by space mutation.The restoring and combining ability of mutants crossing with diverse sterile lines were evaluated.The genetic polymorphism between mutants and wild type was also studied using Simple Sequence Repeat（SSR） primers in rice.The results showed that space mutagenic effects could cause variation of restoring ability in posterity of restorer lines,and pollen sterility of F1 hybrid combinations represented a certain extent variation compared with that of control.Especially,some restorer lines mutated into maintainer lines.The values of GCA of the yield-related characters of R998-3,R998-4,Gui99-2,Gui 99-4 and Hanghui No.7-4 were large.There were 2 538 loci detected by 104 pairs of SSR primers,which distributed over the 12 rice chromosomes.The frequency of polymorphism was 9.85%,which mainly caused by small fragments of DNA deletion or insertion mutation.

关 键 词：水稻 空间诱变 恢复力 变异特性 遗传多态性 

分 类 号：S852.65[农业科学—基础兽医学]