2个籼稻低直链淀粉突变体Wx等位基因序列的研究  

Study on the Wx Allele Gene Sequences of Two Low Amylose Content Mutants of Oryza indica

作　　者：郭涛[1] 韩诗曼[1] 夏斌[1] 王慧[1] 刘永柱[1] 张建国[1] 陈志强[1] 

机构地区：[1]华南农业大学,国家植物航天育种工程技术研究中心,广东广州510642

出　　处：《华南农业大学学报》2011年第3期6-9,共4页Journal of South China Agricultural University

基　　金：广东省自然科学基金(10151064201000058);863计划项目(2007AA1F00101);国家科技支撑计划项目(2008BAD97B02);现代农业产业技术体系建设专项资金资助

摘　　要：根据粳型水稻Wx基因全序列设计引物,扩增2个空间诱变低直链淀粉突变体XLA-1和XLA-2的Wx等位基因.序列分析结果表明:XLA-1、XLA-2与原种籼小占在Wx基因DNA序列上存在较多碱基差异,这种差异以碱基改变为主;XLA-1、XLA-2在第10外显子(+1 208 bp)处存在一个单核苷酸突变(籼小占为碱基T,突变体为碱基C),编码子由TCT突变为CCT,导致丝氨酸突变成脯氨酸,氨基酸的改变可能改变了Wx蛋白的空间结构,致使它不能充分结合在淀粉颗粒上,进而导致直链淀粉含量下降.本研究初步证明在2个籼稻突变体中存在Wx的复等位基因影响直链淀粉含量.Based on the Wx gene sequence of japonica rice,the Wx gene sequences of the two low-amylose mutants XLA-1 and XLA-2 of Oryza indica were detected by PCR and sequencing analysis.The main results showed that： There were many mutant sites among XLA-1,XLA-2 and Xianxiaozhan（the wild type）,most of them were base change.There was a sigle nucleotide polymorphisms（SNP） in the 10th exon（＋1 208 bp） of XLA-1 and XLA-2（Xianxiaozhan for the T,mutant for the C）,codon TCT changed into CCT,which resulted in the substitution of a proline residue for serine.The substitution could alter the spatial structure of Wx protein,which may not be fully integrated on the starch granules,leading to the decreased of amylose content.The preliminary results indicated that the existence of Wx allelles affected amylose content in the two mutants.

关 键 词：籼稻 空间诱变 低直链淀粉突变体 WX基因 序列分析 

分 类 号：S335[农业科学—作物遗传育种]