2个籼稻低直链淀粉突变体淀粉合成关键酶分析  

Activity Assay of Grain Starch Synthesis Key Enzymes in Two Low Amylose Content Mutants from Oryza indica

作　　者：夏斌[1] 郭涛[1] 王慧[1] 刘永柱[1] 张建国[1] 陈志强[1] 

机构地区：[1]华南农业大学,国家植物航天育种工程技术研究中心,广东广州510642

出　　处：《华南农业大学学报》2011年第3期10-13,17,共5页Journal of South China Agricultural University

基　　金：广东省自然科学基金(10151064201000058);863计划项目(2007AA1F00101);国家科技支撑计划项目(2008BAD97B02);现代农业产业技术体系建设专项资金资助

摘　　要：对2个空间诱变低直链淀粉突变体XLA-1和XLA-2灌浆期籽粒4种淀粉合成关键酶ADP-葡萄糖焦磷酸化酶(AGPase)、可溶性淀粉合成酶(SSS)、颗粒结合型淀粉合成酶(GBSS)和淀粉分支酶(SBE)的活性进行了测定.结果表明:原种籼小占与XLA-1、XLA-2的4种淀粉合成关键酶的变化趋势相似,但活性存在差别,除SBE外,原种籼小占的AGPase、SSS和GBSS的活性从整体趋势上都高于XLA-1、XLA-2;在3个供试材料中,AGPase和SSS的活性峰值均早于GBSS、SBE;原种籼小占和XLA-2的直链淀粉积累与GBSS活性呈极显著正相关,而XLA-1的直链淀粉积累与GBSS的活性则呈显著相关;XLA-1与XLA-2的GBSS活性及峰值日期存在明显差异,两者突变机理可能有所不同.The four key enzymes（ADP-glucose pyrophosphorylase（AGPase）,soluble starch synthase（SSS）,granule bound starch synthase（GBSS） and starch branching enzyme（SBE） activity in the mutations XLA-1 and XLA-2 were assayed during the grain filling stages.The main results obtained as follows：There was a similar trend among the four key enzymes of starch synthesis in XLA-1、XLA-2 and control,but they had different activity.The activity of AGPase,SSS and GBSS in control were higher than that in XLA-1 and XLA-2,except SBE.In these tested materials,the peak activity of AGPase and SSS both expressed earlier than that of GBSS and SBE.There was a very significant positive relationship between the activity of GBSS and the accumulation of amylose in control and XLA-2,and a significant positive relationship between the activity of GBSS and the accumulation of amylose in XLA-1.There was an obvious difference in the activity of GBSS and the peak activity between XLA-1 and XLA-2.

关 键 词：籼稻 空间诱变 低直链淀粉突变体 淀粉合成关键酶 

分 类 号：S335[农业科学—作物遗传育种]