培矮64S空间诱变突变株系花药培养条件的探讨  

Studies on Anther Culture Conditions of Space Mutation Lines from Peiai 64S

作　　者：黄翠红[1] 郭涛[1] 刘永柱[1] 张建国[1] 陈志强[1] 王慧[1] 

机构地区：[1]华南农业大学,国家植物航天育种工程技术研究中心,广东广州510642

出　　处：《华南农业大学学报》2011年第3期14-17,共4页Journal of South China Agricultural University

基　　金：863计划项目(2007AA100101);国家科技支撑计划项目(2008BAD97B02);现代农业产业技术体系建设专项资金资助

摘　　要：对培矮64S空间诱变突变株系在培养基选择、激素配比、有机添加物等方面进行了花药培养条件的优化改良.结果表明:N6和M8为较适用于培矮64S突变株系花药培养的培养基,激素则以2,4-D 2.0 mg.L-1+NAA1.0 mg.L-1+KT 0.5 mg.L-1为宜;在此基础上添加水解酪蛋白的培养效果较好,椰汁次之.在诱导植株再生阶段,MS的效果优于N6,表明植株再生需要较高浓度的氨态氮源.To optimum the anther culture conditions for Peiai 64S mutants induced by space,different mediums,such as hormones and organic additives were prepared.The results show that N6 and M8 medium,which contented the combination of hormone in 2,4-D 2.0 mg·L-1 ＋NAA 1.0 mg·L-1＋KT 0.5 mg·L-1,were suitable for the anther culture of Peiai 64S SP2 generation.Adding casein hydrolysate in medium was the best treatment for Peiai 64S,followed by the treatment of adding coconut juice.MS medium was better than N6 medium,which indicated that anther culture materials needed more nitrogen resources in differentiation phase.

关 键 词：水稻 培矮64S 空间诱变 突变株系 花药培养 

分 类 号：S335[农业科学—作物遗传育种]