农杆菌介导的角质细胞生长因子-1转化油菜的研究  

Genetic Transformation of Keratinocyte Growth Factor-1 to Brassica napus Mediated by Agrobacterium tumefaciens

作　　者：杜美丽[1,2] 刘秀明[1,2] 江莺[1,3] 李巍[1,2] 朱海林[1,2] 李海燕[1,2] 李校堃[1] 

机构地区：[1]吉林农业大学,生物反应器与药物开发教育部工程研究中心,吉林长春130118 [2]吉林农业大学生命科学学院,吉林长春130118 [3]吉林农业大学中药材学院,吉林长春130118

出　　处：《华南农业大学学报》2011年第3期73-76,共4页Journal of South China Agricultural University

基　　金：国家863计划(2007AA100503);吉林省科技发展计划重点项目(20070922);高等学校科技创新工程重大项目培育资金项目(70S018)

摘　　要：构建植物表达载体pCAMBIA139035S-KGF1,并将重组质粒转入到根瘤农杆菌EHA105中.以甘蓝型油菜‘沪油15’为受体材料,用农杆菌介导法转入角质细胞生长因子(KGF)-1.通过PCR、Southern blot杂交和Western blot检测,表明KGF-1已经在油菜中成功表达,相对分子质量为19 000.The plant expression vector pCAMBIA139035S-KGF1 was constructed,and transferred to the Agrobacterium tumefaciens EHA105.The keratinocyte growth factor（KGF）-1 gene was transformed into the receptor material Brassica napus ＇Huyou15＇ via Agrobacterium tumefaciens.The transgenic plants were detected by PCR,Southern and Western blot,which showed that KGF-1 gene（Mr=19 000） was expressed successfully in transgenic Brassica napus.

关 键 词：油菜 角质细胞生长因子 转化 

分 类 号：Q786[生物学—分子生物学]