多杀菌素微球制备关键工艺研究:Ⅱ  

Study on key process of preparation of spinosad microsphere:Ⅱ

作　　者：黄彬彬[1] 杨丰梅[1] 张旭溪[1] 钟建斌[1] 吴祖建[1] 吴刚[1] 

机构地区：[1]福建农林大学植物保护学院,福州350002

出　　处：《农药学学报》2011年第4期402-408,共7页Chinese Journal of Pesticide Science

基　　金：福建省科技厅项目(2009N00182009J01071)

摘　　要：采用乳化-溶剂挥发法,以聚乳酸(PLA)为成球材料(壁材)制备了多杀菌素微球。研究了PLA浓度和油/水相体积比对多杀菌素微球制备的影响规律,确定了制备多杀菌素微球的优选配方及工艺条件。制备得到中位径(D50)为12.73μm、跨距为1.4811、载药量在31%左右、包封率为100.2%、包封产率为89.4%的多杀菌素微球,重复性良好。扫描电镜(SEM)观察结果表明,所得微球为表面较光滑的实心小圆球。差示扫描量热(DSC)分析结果证实,多杀菌素和PLA的确形成了载药微球。室内毒力测定结果表明,自制5%多杀菌素微球悬浮剂与市售2.5%多杀菌素悬浮剂(菜喜)对小菜蛾P lutella xylostella2龄幼虫的毒力基本相同,LC50值分别为0.40和0.38μg/mL。Spinosad was prepared into microsphere using polylactic acid（PLA） as coating material（wall material） by emulsion solvent evaporation method.The optimum prescription and process conditions were determined by investigating the effects of concentration of PLA and ratio of oil phase volume to water phase volume on quality of preparing spinosad microsphere.The result showed that D50,span,drug-loading rate,entrapment rate,drug yield of the spinosad microsphere is 12.73 μm,1.481 1,31%,100.2% and 89.4% respectively and this preparation process has a good repeatability.Spinosad microspheres were observed by scanning electron microscope（SEM）,the result showed that the microspheres are round beads which have relatively smooth surfaces.The result of analyzing differential scanning calorimetry（DSC） confirms that spinosad and PLA had formed drug-loaded microphere indeed.The determination result of laboratory toxicity showed that the bioactivity of self-made 5% suspension agent of spinosad microspheres and 2.5% spinosad suspension agent sold in the market against 2nd-instar larvae of Plutella xylostella are almost the same,LC50 value was 0.40 and 0.38 μg/mL,respectively.

关 键 词：多杀菌素 聚乳酸 微球制备 

分 类 号：TQ450.62[化学工程—农药化工]