聚合物纳米微球分离纯化格尔德霉素方法研究  

Studies on Separation and Purification of Geldanamycin by Nanoscale Polymer Particles

作　　者：李宁[1] 张雪霞[1] 李晓露[1] 王海燕[1] 林毅[1] 蒋沁[1] 

机构地区：[1]微生物药物国家工程研究中心河北省工业微生物代谢工程技术研究中心华北制药集团新药研究开发有限责任公司,河北石家庄050015

出　　处：《化学与生物工程》2011年第7期74-76,共3页Chemistry & Bioengineering

基　　金：河北省科技支撑计划项目(09246407D)

摘　　要：研究了聚合物纳米微球分离纯化格尔德霉素的方法。通过聚合物纳米微球型号、洗脱液体积分数、洗脱速度、加样量的选择,确定聚合物纳米微球分离纯化格尔德霉素工艺为:采用PS30RPC型聚合物纳米微球作为层析填料,洗脱液乙醇体积分数65%、洗脱速度6.0 mL.min-1、加样量(样品∶填料,g∶g)1∶100,此时,平均层析收率为92.5%、平均总收率为81.6%、平均成品纯度为98.7%。该分离纯化方法简便可靠,适于工业化生产。The method of separation and purification of geldanamycin by nanoscale polymer particles was studied.The conditions for separation and purification of geldanamycin by nanoscale polymer particles were optimized,by choosing the type of nanoscale polymer particles,the eluent volume fraction,the elution speed and the amount of sample.Type of PS30RPC nanoscale polymer particles was selected as chromatography filler.Under the optimal conditions as follows： using 65% ethanol as eluent,the amount of sample was 1∶100（sample∶filter,g∶g）,and the elution speed was 6.0 mL·min-1,the even elution yield was 92.5%,the even total yield was 81.6% and the even purity of the final product was 98.7%.The simple and reliable method of separation and purification of high-purity geldanamycin is suitable for industrial production.

关 键 词：聚合物纳米微球 格尔德霉素 纯化 

分 类 号：Q503[生物学—生物化学] R979.1[医药卫生—药品]