高效液相色谱法同时测定静脉镇痛液中盐酸吗啡和盐酸曲马多及柠檬酸芬太尼与氯诺昔康的含量  

Simultaneous determination of morphine, tramadol, fentanyl and lornoxicam in analgesic mixture samples by high performance liquid chromatography

作　　者：陈富超[1] 方宝霞[1] 丁洁[2] 李鹏[1] 时晓亚[1] 董敏[1] 

机构地区：[1]湖北医药学院附属东风医院药学部,湖北省十堰市442008 [2]湖北医药学院附属东风医院麻醉科,湖北省十堰市442008

出　　处：《中国医药》2011年第8期960-961,共2页China Medicine

基　　金：湖北省十堰市科学技术研究与开发计划项目（20lOst45）

摘　　要：目的 建立使用高效液相色谱法（HPLC）同时测定静脉镇痛液中盐酸吗啡、盐酸曲马多、柠檬酸芬太尼与氯诺昔康含量的方法.方法 在Kromasil C18（150mm×4.6mm,5μm）色谱柱,甲醇-0.05 mol/L磷酸二氢钾为流动相,梯度洗脱,流速为0.8 mL/min的色谱条件下,同时测定静脉镇痛液中盐酸吗啡、盐酸曲马多、柠檬酸芬太尼与氯诺昔康含量,以探讨此HPLC的检测效果.结果 在上述色谱条件下,盐酸吗啡、盐酸曲马多、柠檬酸芬太尼与氯诺昔康的保留时间分别为5.0、12.3、20.7、23.0 min.各色谱峰理论塔板数均不低于2000,分离度均＞1.5,各色谱峰均为纯峰.精密度检测示,上述各药物的峰面积积分值相对标准偏差（BSD）分别为0.53%、0.35%、0.71%、0.28%.重复性试验示,各峰面积积分值BSD均＜2%.辅料在上述色谱条件下不干扰样品的测定.盐酸吗啡、盐酸曲马多、柠檬酸芬太尼与氯诺昔康分别在5～250 mg/L、4 ～800 mg/L、1～50mgL与4～400 mg/L浓度范围与峰面积具有良好的线性关系;加样回收率分别为99.6%、100.2%、101.1%与99.8%;相应RSD分别为1.43%、0.72%、1.25%与0.88%.结论 本方法 方便、快速、准确,可以作为盐酸吗啡、盐酸曲马多、柠檬酸芬太尼与氯诺昔康配伍检测的方法.Objective To establish a high performance liquid chromatography（HPLC） method for simultaneous determination of the content of morphine, tramadol, fentanyl and lornoxicam in analgesic mixture samples used in intravenous fluids. Methods For simultaneous determination of the content of morphine, tramadol, fentanyl and lornoxicam in analgesic mixture samples, the chromatographic separation was performed on a Kromasil C18 （150 mm ×4.6 mm, 5 μm） column using gradient elution, the mobile phase consists of 0. 05 mol/L potassium dihydrogen phosphate in methanol at a flow rate of 0.8 ml/min to evaluate the efficiency of HPLC. Results With above chromatography conditions, the retention times of morphine, tramadol, fentanyl and lornoxicam were 5.0, 12.3, 20.7, 23.0 min, respectively. All peaks were single peak. The theoretical plates were no less than 2000 and resolutions were more than 1.5. By precision experiments, the relative standard deviation（RSD） of peak areas in morphine, tramadol, fentanyl and lornoxicam were 0.53% , 0.35% , 0.71% , 0.28%. By reproduction experiments, the RSD of peak areas were less than 2%. The adjuvants cannot interfere with the sample determination under above separation conditions. The good linear relationships of morphine, tramadol, fentanyl and lornoxicam were obtained in the ranges of 5-250 mg/L, 4-800 mg/L, 1-50 mg/L and 4-400 mg/L, respectively; the average recoveries of morphine, tramadol, fentanyl and lornoxican were 99. 6%, 100. 2%, 101. 1%and 99. 8%, respectively. RSDs were 1.43%, 0.72%, 1.25% and 0. 88%. Conclusion The method is sensitive, rapid and accurate. It＇s suitable for compatible determination of morphine, tramadol, fentanyl and lornoxicam in analgesic mixture samples.

关 键 词：色谱法 高压液相 吗啡 曲马多 芬太尼 氯诺昔康 

分 类 号：R917[医药卫生—药物分析学]