蜕皮甾酮和骨髓间充质干细胞对早期创面抗炎作用的初步研究  

An experimental research on anti-inflammatory effect of ecdysterone and bone mesenchymal stem cells at early period of burn wound

作　　者：张子良[1] 吴旭[1] 张军花[1] 严智敏[1] 张元中[1] 冯长江[1] 付小兵[2] 

机构地区：[1]南方医科大学南方医院,广东广州510515 [2]解放军总医院第一附属医院全军创伤修复与组织再生重点实验室,北京100048

出　　处：《感染．炎症．修复》2011年第2期81-84,I0002,共5页Infection Inflammation Repair

基　　金：广东省科技计划项目(2008B010600006)

摘　　要：目的:研究蜕皮甾酮(EDS)及骨髓间充质干细胞(BMSC)对烫伤创面的早期抗炎作用。方法:新西兰大耳白兔18只,每只兔用烫伤仪制备4个创面并经病理证实为深Ⅱ度,随机标记为空白对照组(A组)、EDS组(B组)、BMSC(C组)、EDS+BMSC(D组)。烫伤后5、10、15 d各处死6只家兔,观察炎性细胞浸润度、创面愈合率和组织病理学评分的变化。结果:烫伤后5 d,B、D组炎性细胞浸润度明显示低于A组(P<0.05),C组变化不明显,B组创面愈合率明显高于A组(P<0.05);烫伤后10 d,各组各指标差异无显著性;烫伤后15 d,各组炎性细胞浸润度差异无显著性,B、C、D组创面愈合率、组织病理学评分均高于A组(P<0.05或P<0.01)。结论:EDS对家兔烫伤皮肤有早期抗炎作用,这可能是EDS促进创面愈合的一种机制。EDS联合BMSC亦具有早期抗炎作用,两者联合不存在拮抗作用,是否存在叠加作用还需进一步研究。Objective： To investigate the anti-inflammatory effect of ecdysterone （EDS） and bone mesenehymal stem cells （BMSC） at early period of burn wound in rabbits. Methods： Scald injury was reproduced in 18 rabbits by scald instrument, and 4 scald wounds of deep second degree were made on skin, and they were divided randomly to the groups of A, B, C and D. Wounds in group A was blank control group, group B was treated with EDS, group C with BMSC, and group D with BMSC＋EDS. Six rabbits were sacrificed to obtain the histological specimens on 5, 10, 15 days （nz 6）, respectively, after the treatment. The degree of infiltration of inflammatory cells, histopathological scores and wound healing rate were observed to assess the therapeutic effect. Results： Five days after scald, the degree of inflammatory cell infiltration of group B and D was lower than group A （P〈0.01）, while no markedly changes were found in group C; the wound healing rate of group B was obviously higher than that of the group A （P〈0.05）. On 10th day after scald, there were no significant differences among all groups in the degree of infiltration of inflammatory cells, wound healing rate and histopathological scores（P〈0.05）. On 15th day after scald, there were no significant differences among all groups in the degree of infiltration of inflammatory cells （P〈0.05）, but the wound healing rates and histopathological scores of group B, C and D were obviously higher than that of group A （P〈0.05 or P〈0.01）. Conclusion： EDS can enhance anti-inflammatory effect at an early period of scald wound in rabbits. It may be a mechanism of enhancing wound healing of EDS application. When EDS is combined with BMSC, the anti-inflammatory effect at early period of scald wound is enhanced. There is no antagonism between two agents, but the additive effects of them is not confirmed and warrants more research work.

关 键 词：蜕皮甾酮 骨髓间充质干细胞 抗炎 烫伤 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]