血管内放射对猪冠状动脉球囊损伤后细胞增殖和凋亡的影响  

EFFECTS OF ENDOVASCULAR RADIATION ON CELL PROLIFERATION AND APOPTOSIS AFTER CORONARY ARTERY BALLOON INJURY IN SWINE

作　　者：何昆仑[1] 盖鲁粤[1] 黄大显[1] 王所亭[1] 郭爱桃[1] 高焱章[1] 

机构地区：[1]北京解放军总医院,100853

出　　处：《解放军医学杂志》1999年第6期423-425,共3页Medical Journal of Chinese People's Liberation Army

基　　金：国家自然科学基金!资助课题(编号 39900059)

摘　　要：探讨血管内放射抑制冠状动脉内介入治疗后再狭窄的机制。通过小型猪冠状动脉球囊扩张术后再狭窄动物模型，采用免疫组织化学方法，观察了血管内放射治疗对细胞增殖和细胞凋亡的影响。发现血管内放射抑制了球囊扩张术后３ 天血管外膜和中膜的细胞增殖，同时也抑制了术后３０ 天血管内膜的细胞增殖，但对术后３ 天和３０ 天细胞凋亡无显著影响。血管内放射可能通过抑制球囊扩张术后早期外膜和中膜以及晚期内膜的细胞增殖而预防再狭窄。This study examined the effects of endovascular irradiation on cell proliferation and apoptosis after overstretch balloon angioplasty in a normolipemic swine model of restenosi. Twenty five swine underwent oversized balloon angioplasty in the LAD and/or LCX,and in 13 of them the procedure was followed immediately by endovascular radiation using a guide wire with 3 cm long 192 Ir line source to deliver 20Gy to overdilated segments. All the animals were killed at 3 and 30 days. Cell proliferation was estimated by immunostaining for proliferation cell nuclear antigen (PCNA). Apoptosis was identified by the terminal uridine nick end labeling(TUNEL). The results showed that endovascular radiation significantly reduced the pcna positive cells compared with controls 3 days and 30 days after initial injury. There were no quantitative differences in the amount of TUNEL labeling cells among irradiated and control vessels in any region examined 3 days and 30 days after balloon injury. Endovascular radiation may reduce restenosis by inhibiting the cell proliferation, not by enhancing cell apoptosis.

关 键 词：血管成形术 冠状动脉 球囊损伤 介入疗法 猪 

分 类 号：R654.2[医药卫生—外科学]