濒危药用植物天山雪莲的根段组织培养与植株再生  

In Vitro Micro-propagation of Saussurea Involucrata an Endangered Chinese Medicinal Herb

作　　者：江丽丽[1] 赵红艳[2] 马淼[1] 

机构地区：[1]石河子大学生命科学学院,石河子832003 [2]新疆师范大学生命科学学院,乌鲁木齐830054

出　　处：《石河子大学学报（自然科学版）》2011年第3期343-346,共4页Journal of Shihezi University(Natural Science)

基　　金：国家自然科学基金项目(30960028)

摘　　要：以濒危药用植物天山雪莲根段为研究材料进行组织培养和植株再生研究,旨在拓宽天山雪莲组织培养外植体的范围,建立高效植株再生体系,为其种群复壮、资源保护与可持续利用提供技术参考。研究结果表明:适合天山雪莲根段愈伤组织诱导的最佳培养基为MS+2,4-D(0.5 mg/L)+NAA(2.0 mg/L),愈伤组织的诱导率为100%;适合其根段愈伤组织丛生芽分化的最佳培养基为MS+NAA(0.05 mg/L)+6-BA(0.4 mg/L),分化率为42.6%,增殖倍数为4.3;最佳生根培养基为1/2 MS+NAA(0.3 mg/L),在此条件下,根发育良好,生根率为76.9%,植株健壮;组培苗炼苗后移栽,成活率可达89%。An efficient micro-propagation system for Saussurea involucrata Kar.et Kir.an endangered Chinese medicinal plant,has been developed.100% callus formation frequency was got from root explants inoculated on a MS medium containing 0.5 mg/L 2,4-D and 2 mg/L NAA（1-Naphthaleneacetic acid）.42.6% of adventitious bud organogenesis frequency and 4.3 buds per explant were got from root-derived callus when cultured on a MS medium supplemented with 0.05 mg/L NAA,0.4 mg/L 6-BA.Up to 76.9% of the regenerated shoots formed complete roots on a half-strength MS medium containing 0.3 mg/L NAA,and 89% of the complete plantlets survived and grew vigorously under greenhouse condition.

关 键 词：天山雪莲 愈伤组织 丛生芽 植株再生 濒危植物 

分 类 号：R944.4[医药卫生—药剂学]