同步提取棉花DNA和RNA的方法  

A Method of Simultaneous Isolation of DNA and RNA from Cotton

作　　者：朱新霞[1] 艾尼江 闫洁[1] 赵海 

机构地区：[1]石河子大学生命科学院,石河子832000 [2]新疆石河子棉花所,石河子832000

出　　处：《石河子大学学报（自然科学版）》2011年第3期347-351,共5页Journal of Shihezi University(Natural Science)

基　　金：国家科技部农业科技成果转化基金项目(2010GB2G410602)

摘　　要：为节约样品数量、简捷快速地提取棉花DNA与RNA,以棉花幼苗为研究对象,采用改进的CTAB法,进行了同步提取棉花DNA与RNA的方法研究。通过电泳检测、含量测定和SSR、AFLP、RT-PCR、cDNA-AFLP等对得到的DNA与RNA进行了质量验证。结果表明:该方法简便、快速、同步提取的DNA及RNA质量好,能满足后续分子生物学试验的要求。In order to save sample quantity,DNA and RNA can be isolated from cotton at the same time,CTAB method was improved in this paper.The total DNA and RNA isolated by this method are of sufficient quality for the following molecular biology research such as SSR,AFLP,RT-PCR,cDNA-AFLP and so on.Thus,this method established a good foundation for the successive molecular experiments.

关 键 词：棉花 DNA RNA 同步提取法 

分 类 号：Q933[生物学—微生物学]