牙龈卟啉单胞菌感染对血管内皮细胞LOX-1 mRNA表达的影响  

Expression of LOX-1 mRNA in Vascular Endothelial Cells Infected by Porphyromonas Gingivalis

作　　者：王生[1] 蔡志斌[2] 张大风[2] 邓辉[2] 

机构地区：[1]解放军第一一八医院口腔科,浙江温州325000 [2]温州医学院附属口腔医院口腔内科

出　　处：《广东牙病防治》2011年第7期353-356,共4页Journal of Dental Prevention and Treatment

基　　金：温州市科技计划项目(Y20060110);浙江省高校科研计划(Y200803098)

摘　　要：目的研究牙龈卟啉单胞菌(Porphyromonas gingivalis,P.g)感染对血管内皮细胞(vein endothelial cell,VEC)血凝素样氧化低密度脂蛋白受体-1(lectin-like oxidized low-density lipoprotein receptor-1,LOX-1)mRNA表达的影响。方法建立P.g感染VEC的体外模型,采用Real Time-PCR技术检测P.g381菌株感染人脐静脉内皮细胞(human umbilical vein endothelial cells,HUVEC)细胞株EA.hy926不同时间后,LOX-1 mRNA表达的变化。结果 P.g381侵入HUVEC促进LOX-1 mRNA的表达,与对照组相比较(△Ct值为3.78±0.16),2 h时开始升高(△Ct值为3.60±0.33,P=0.240),8 h达高峰(△Ct值为2.72±0.35,P=0.000),12 h后显著回落(△Ct值为3.22±0.17,P=0.001),至24 h时恢复至最初水平(△Ct值为3.65±0.24,P=0.390)。结论 P.g感染促进HUVEC表达LOX-1 mRNA。Objective To investigate the ability of Porphyromonas gingivalis（P.g） to modulate the expression of LOX-1 in endothelial cells linked to periodontal infections and proatherogenic pathways.Methods A cell culture model of human umbilical vein endothelial cells（HUVEC） was used in vitro.EA.hy926 was infected by P.g381 for 2 h,8 h,12 h and 24 h.Then the expression of LOX-1 mRNA were measured by Real-time PCR.Results P.g381 infection significantly enhanced the expression of LOX-1 mRNA.Compared to the control,the relative LOX-1 mRNA expression of HUVEC invaded by P.g381 at 2 h,8 h,12 h and 24 h was 113.0%,208%（P0.01）,147%（P0.05）,109%,respectively.Conclusion These data demonstrate that P.g infection enhanced the expression of LOX-1mRNA inHUVEC and may affect the uptake of lipid.

关 键 词：牙龈卟啉单胞菌 血管内皮细胞 LOX-1 

分 类 号：Q939.1[生物学—微生物学]